background and aims: coxsackievirus b3 (cvb3) is the most common agent known to cause viral myocarditis. the viral genome encodes a single polyprotein that is cleaved to produce several proteins by virally encoded proteases. most of this proteolytic processing is catalyzed by a cysteine protease called 3c. the 3c protease plays major role in viral replication and cellular damage. methods: to un...

The anti-Trypanosoma cruzi activity of extracts from 13 Argentinean Asteraceae species was determined. Dichloromethane and methanol extracts of Acmella bellidioides, Aspilia silphioides, Viguiera tuberosa, Calyptocarpus biaristatus, Hyalis argentea, Helenium radiatum, Gaillardia megapotamica, Verbesina subcordata, Gymnocoronis spilanthoides, Viguiera anchusaefolia, Thelesperma megapotamicum, Ze...

introduction: leishmania tarentolae (l. tarentolae) is a nonpathogenic species of leishmania genus that can be used as an expression system to produce immunogenic proteins or epitopes in vivo acting as an efficient and safe recombinant live vector in vaccinology. cysteine proteases (cps) are one group of family c1 peptidases in leishmania that are important for survival of the parasite within t...

in the present work, a new branch of biotechnological advantage of the latex of an endemic perennial plant, euphorbia microsciadia has been introduced. a novel cysteine protease, designated as microsciadin, was purified from the latex of euphorbia microsciadia by a combination of sequential usage of sp-sepharose fast flow column in two different phs and a final gel filtration chromatography. mi...

proteases catalyze the hydrolysis of peptide bonds in proteins. they are widely distributed in nature, nearly in all plants, animals and microorganisms. plant latex is a rich source of proteases. latex production is unique property of euphorbian plant, which could be a potential source of proteolytic enzymes. the highest proteolytic activity was observed in the latex of pedilanthus tithymaloide...

The nonsteroidal antiestrogen EM-800 is approximately 10-fold more potent than ICI 182 780, the most potent known steroidal antiestrogen, at inhibiting estrone-stimulated uterine weight in ovariectomized mice (half-maximal inhibitory daily s.c. doses of 0.2 and 2.0 microg, respectively). At maximal doses, however, both compounds lead to a similar maximal 90% inhibition of estrone-stimulated ute...

Trichilia monadelpha (Thonn) JJ De Wilde (Meliaceae) bark extract is used in African traditional medicine for the management of various disease conditions including inflammatory disorders such as arthritis. The present study was undertaken to evaluate the anti-inflammatory properties of aqueous (TWE), alcoholic (TAE) and petroleum ether extract (TPEE) of T. monadelpha using the 7-day old chick-...