An extremely haloalkaliphilic archaeon Natrinema sp. SSBJUP-1 showing potent amylolytic and proteolytic activities was isolated from the saline alkaline Lonar Lake, India. Amylase and protease were active over the neutral to alkaline pH range from 6 to 9. Amylase was more stable over a narrower temperature range of 40-60C than the protease but had the higher optimum (55C) for activity. By contr...

Proteases are one of the largest and best-characterized families of enzymes in the human proteome. Unfortunately, the understanding of protease function in the context of complex proteolytic cascades remains in its infancy. One major reason for this gap in understanding is the lack of technologies that allow direct assessment of protease activity. We report here an optimized solid-phase synthes...

A novel peptide substrate (A G G P L G P P G P G G) was developed for quantifying the activities of bacterial enzymes using a highly sensitive Fluorescence Resonance Energy Transfer (FRET) based assay. The peptide substrate was cleaved by collagenase class I, II, Liberase MTF C/T, collagenase NB1, and thermolysin/neutral protease, which was significantly enhanced in the presence of CaCl2. Howev...

One hundred and fifty-one Cryptococcus neoformans strains isolated from AIDS patients in Brazil and maintained in the Adolfo Lutz Institute (São Paulo, Brazil) were tested for phospholipase, protease and other extracellular enzymatic activities and their serotypes determined. Production of extracellular phospholipase and protease was tested by the agar plate methods. Determination of extracellu...

The hepatitis C virus (HCV) nonstructural 3 protein (NS3) is a 70-kDa multifunctional enzyme with three known catalytic activities segregated in two somewhat independent domains. The essential machinery of a serine protease is localized in the N-terminal one-third of the protein, and nucleoside triphosphatase (NTPase) and helicase activities reside in the remaining C-terminal region. NS4A is a ...

Natural Antimicrobial peptides (AMPs) are important components of immune systems and possess immuno-modulatory and broad spectrum antimicrobial activities. These host-defense peptides failed to resist protease degradation or to efficiently inhibit biofilm formation. We describe in this review the different strategies to improve AMPs and develop peptides that could be used clinically and in indu...

The protein-ligand binding interactions studies were carried out by performing dockings of the ligands that were found to be competitively inhibiting the activities of the DEN2 NS2B/NS3 serine protease onto the catalytic triad of a model of DEN2 NS2B/NS3 protease. Results indicate the importance of three out of the five residues reported to be essential for binding activities of the NS2B/NS3 se...

Conditions are described for the production of large amounts of an extracellular elastolytic protease by Vibrio vulnificus. The yield of enzyme was maximal during the late exponential growth phase and was stable during the stationary growth phase in a medium composed of 2% Proteose Peptone and 1.5% NaCl. The protease has a molecular weight of ca. 50,500 (estimated by sodium dodecyl sulfate-poly...

the protease enzyme breaks down protein into amino acids as its constructors. protease immobilization onto appropriate support materials plays an essential role in various fields of technology including the food and detergent industries. accordingly, improvement of protease immobilization has been highly regarded due to its applications in bio catalysis. alginate and chitosan are natural polysa...