Organogenesis and neoformant plantlet production were studied by culturing the different parts of mature embryo including radicle and proximal and distal parts of cotyledon of Mission and Kroneiki cultivars. Different parts of embryos were cultures in OMc medium containing growth regulators, such as indole butyric acid (IBA) and 2-isopentenyl adenine (2-ip) or IBA and naphthalene acetic acid (N...

As both major macronutrients and signal molecules, nitrogen metabolites, such as nitrate and nitrite, play an important role in plant growth and development. In this study, the callus growth of indica rice cv. 9311 was significantly enhanced by nitrite, whereas the soluble protein content remained unchanged. The deep RNA sequencing technology (RNA-seq) showed that the transcriptional profiles o...

This research was undertaken for optimizing simple and reproducible protocol on indirect regeneration in tomato using kinetin. The effect of kinetin for efficient callus production was studied and its results were interpreted. Hypocotyls and cotyledons were used as explants and effect on callus derived regeneration was greatly influenced by addition of kinetin in media. The individually positio...

Agrobacterium-mediated genetic transformation was explored for inducing insect resistance in mat rush (Juncus effusus L.). Firstly, the callus regeneration system was established, and secondly the Agrobacterium tumefaciens strain EHA105 with plasmid pKUB carrying cryIA(b), hph and gus genes, coding for hygromycin phosphotransferase and β-glucuronidase, respectively, was used for transformation....

Having produced the embryos of cactus Copiapoa tenuissima Ritt. forma monstruosa at the globular stage and callus, we investigated the effect of abscisic acid (ABA) in the following concentrations: 0, 0.1, 1, 10, and 100  μ M on successive stages of direct (DSE) and indirect somatic embryogenesis (ISE). In the indirect somatic embryogenesis process we also investigated a combined effect of ABA ...

plant regeneration was achieved in verbascum speciosum schard. via organogenesis and somatic embryogenesis by culture of mature embryo explants. two types of calli, embryogenic and non-embryogenic, were induced from mature embryo explants on murashige and skoog (ms) medium supplemented with different concentrations of benzyl adenine (ba) and α-naphthalene acetic acid (naa). in order to further ...

Asparagus breslerianus a wild species in Iran, exhibited tolerance to salt in dry gypsum hills and dry lands. In order to check for salt tolerance thresholdvia in vitro conditions, the A. breslerianus callus was subjected to NaCl (sodium chloride) treatments. Six weeks old calli derived from male spear bud, were exposed to 0, 21.88, 43.76, 65.64, 76.58, 87.52, 109.40, 131.28, 153.16 and 175.04 ...

Unavailability of performant planting material pineapple constitutes a major problem its cultivation in Africa. For this purpose, indirect organogenesis technique is used to evaluate the vitro responses two cultivars during explant’s regeneration. Calli were induced from crown leaf and plantlets “Smooth Cayenne” “Sugarloaf cultivars”. Murashige Skoog medium with vitamins B5 supplemented differe...

A simple micropropagation protocol has been achieved using nodal region and apical leaf of Clerodendrum indicum,(L) kuntze; an important medicinal plant of Verbenaceae. The explants were cultured in MS medium supplemented with NAA, BA, 2,4-D,Kn, IBA in various combination and concentrations (0.110.0 μM). Response to callogenesis were diverse, ranging from white vigorous compact callus (1.0μM NA...

Preharvest sprouting of wheat (Triticum aestivum L.) is associated with inadequate seed dormancy. Although abscisic acid (ABA) has often been suggested to play a central role in developing seed, its involvement in dormancy of mature seed lacks firm experimental evidence and endogenous ABA levels are not well correlated with germinability. We examined genotypic and temporal variation in wheat se...