This study investigated the effect of spirulina ethanolic extract (SEE) on quality ram semen during low-temperature liquid storage and relationship between sperm features. Ejaculates were collected from five Djallonké rams, pooled, extended with Tris-egg yolk (TEY) enriched 0 (control), 20 (SEE20), 40 (SEE40), 80 µg/mL (SEE80) SEE to reach concentration 200×106 spz/mL, stored at 4 °C for 72 h. ...

AIM To investigate the distribution of cation channel of sperm 1 (CATSPER1) protein and the presence of CATSPER1 mRNA in human testis and ejaculated spermatozoa. The influence of anti-human CATSPER1 antibody upon human sperm motility was used to evaluate the function of human CATSPER1 and to estimate its possible use as a target for immunocontraception. METHODS Human ejaculated sperm from nor...

Background: Obesity and raised body mass index (BMI) is a major risk factor for several diseases such as cardiovascular disease, type 2 diabetes, as well as gynecological problems. In recent years, some studies have associated the BMI with reproductive parameters in men, showing that increased BMI is related to poor semen quality, decreased sperm concentration, decreased and normal-motile sperm...

background: the lipid peroxides and their degradation products are highly toxic to spermatozoa and may play a major role in sperm dysfunction. the purpose of this study was to determine the level of lipid peroxidation as indicated by malondialdehyde (mda) in the spermatozoa and seminal plasma of asthenozoospermic and normozoospermic males. in addition, correlation of lipid peroxidation with mot...

background: spermatozoa cryopreservation is used for the management of infertility and some other medical conditions. the routinely applied cryopreservation technique depends on permeating cryoprotectants, whose toxic effects have raised the attention towards permeating cryoprotectants-free vitrification technique. objective: to compare between the application of slow cryopreservation and vitri...

Background: Diabetes mellitus is an endocrine disorder of carbohydrate metabolism resulting primarily from inadequate insulin release (type I) or insulin insensitivity coupled with inadequate compensatory insulin release (type II). Oxidative stress is one of the complications of diabetes that increases in both types of diabetes and treatment with antioxidants can reduce the complications of dia...

background: sperm selection is generally used to describe methods for separation of spermatozoa for in-vitro-assisted reproduction. few studies reported relation in vivo fertility and semen parameters after selection of fertile spermatozoa; hence, our research attempt to assess different semen parameters after post-thaw (pt) or after selecting sperm sample (ssp), using density gradient separati...