25(OH) D3. Finally, the renal 1α-hydroxylase converts 25(OH) D3 to 1,25(OH) 2D3, which is the most biologically active metabolite of VD (Figure 1).3 The actions of 1, 25(OH) 2D3 are mediated by binding to its high-affinity receptor, the VDR. Furthermore, the cellular response to VD is not only dependent on VDR, but also on presence and activity of VD metabolizing enzymes. Previous studies sugge...

Sixty semen ejaculates from 10 mature bulls, 5 each of Jafarabadi and Mehsana breed, were studied for sperm motility and velocity parameters of fresh and frozen-thawed spermatozoa using computer assisted sperm analyzer (CASA). The mean values of motile and progressively motile spermatozoa observed in fresh semen of Jafarabadi and Mehsana bulls (79.77±1.62 and 61.80±1.85, and 78.90±1.22 and 61.3...

s / Journal of Equine Veterinary Science 34 (2014) 76 76 x106sperm/mL. Sperm recovery rate after centrifugation was lower in low concentration samples (p<0.001). Mean lactate concentration at T0 was the same in all three groups and directly correlated with raw semen volume (r1⁄40.5032; p1⁄40.0103). Lactate concentrations at T0 and T24 were not correlated. Compared to T0, lactate concentration w...

introduction: to evaluate the quick cryopreservation technique on parameters of motility, morphology, and vitality of spermatozoa from normozoospermic ejaculates. materials and methods: in this prospective study, a total of 37 normal ejaculates were evaluated according to who criteria, then each sample was divided into two aliquets and sample was cryopreserved with quick freezing method. sperm ...

Factual information for the use of the doctor called upon to give evidence in court after examining the victim of an alleged sexual attack is considered on the basis of an extensive review of the literature and of years of experience by the staff of the Attorney-General's Laboratory for Ontario. This experience emphasizes the need for immediate examination of fluid from the vaginal pool for mot...

The presence of a complex population of mRNAs in human mature spermatozoa is well documented; among them, transcripts of aromatase and ERs (oestrogen receptors) have been described but their significance is not clear. Therefore, to clarify the role of this complex population of mRNAs in human ejaculated sperm, we have isolated on discontinuous density gradients two main fractions from the same ...

The British Andrology Society guidelines for the assessment of post vasectomy semen samples recommend that initial assessment is undertaken 16 weeks post vasectomy and after the patient has produced at least 24 ejaculates. The laboratory should examine a freshly produced seminal fluid specimen by direct microscopy and if no sperm are seen the centrifugate should be examined for the presence of ...

In this contribution we investigate the applicability of photon correlation techniques as a clinical tool for the evaluation of motility parameters of undiluted human sperm. Measurements on semen samples, selected visually for 'good' motility, yielded reproducible estimates of the fraction motile spermatozoa and the mean speed of the motile cells.

Motility is an essential characteristic of all flagellated spermatozoa and assessment of this parameter is one criterion for most semen or sperm evaluations. Computer-aided sperm analysis (CASA) can be used to measure sperm motility more objectively and accurately than manual methods, provided that analysis techniques are standardized. Previous studies have shown that evaluation of sperm subpop...

The post-coital test involves direct microscopic examination of sperm number and motility in cervical mucus. The results depend on the quality of the mucus and the distribution of spermatozoa within the sample. To progress from such qualitative data to quantitative measurements of the spermatozoa present in post-coital mucus, we have developed methods to measure sperm concentrations in enzymati...