OBJECTIVES It was aimed to investigate the effects of different doses of follicle stimulating hormone (FSH) and activin A on the growth and maturation of preantral mouse follicles during the in vitro culture. METHODS Preantral follicles (90-100 microm in diameter) were harvested from 6-8 week-old Syrian mice and cultured in TCM199 culture medium for 6 days to see the effect of FSH and Activin...

This study aims to investigate the effects of follicle stimulating hormone (FSH) and fibroblast growth factor-2 (FGF-2) on the survival and growth of caprine preantral follicles. Ovarian tissues were cultured for 1, 7, 14, 21 or 28 days in medium supplemented with FSH (FSH-2d or FSH-7d, i.e., with replacement of the culture medium every 2 or 7 days, respectively) or FSH+FGF-2 (replacement of th...

Mammalian oocytes grow within ovarian follicles in which the oocyte is coupled to surrounding granulosa cells by gap junctions. We report here that growing oocytes isolated from mouse preantral follicles are incapable of recovering from an experimentally induced acidosis, and that oocytes acquire the ability to manage acid loads by activating Na(+)/H(+) exchange during growth. By contrast, gran...

Activin, a member of the transforming growth factor-β superfamily, promotes the growth of preantral follicles and the proliferation of granulosa cells. However, little is known about the role of microRNAs in activin-mediated granulosa cell proliferation. Here, we reported a dose- and time-dependent suppression of microRNA-181a (miR-181a) expression by activin A in mouse granulosa cells (mGC). O...

Purpose The aim of the study was to evidence whether Thyrotropin (TSH) and Thyroid hormones (T3 and T4) have an impact in in vitro follicle growth of mouse preantral follicles, in in vitro fertilization and in early embryo development. Methods This prospective observational animal experimental study was conducted on a study population consisted of 100 female and 30 male mice (C57BL/6 x CBA) F1 ...

background: this study was conducted to evaluate fibroblast co-culture and activin a on in vitro maturation and fertilization of mouse preantral follicles. methods: the ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-mem as based medium for 12 days. a total number of 456 follicles were cultured in four conditions: (i) base mediu...