AIM In this study, we employed chimeric human/mouse Proteinase 3 (PR3) proteins as tools to induce an autoantibody response to PR3 in rats and mice. METHOD Rats and mice were immunised with recombinant human PR3 (HPR3), recombinant murine PR3 (mPR3), single chimeric human/mouse PR3 (HHm, HmH, mHH, mmH, mHm, Hmm) or pools of chimeric proteins. Antibodies to mPR3 and HPR3 were measured by ELISA...

Wegener's granulomatosis (WG) is an inflammatory disorder characterized by granulomatous inflammation and vasculitis, and is strongly associated with antineutrophil cytoplasmic antibodies (ANCA). ANCA in patients with WG are directed against proteinase 3 (Pr3) in most of the cases. In vitro, upon neutrophil priming, ANCA antigens are expressed on the cell surface, thereby becoming available for...

Alternating current electroluminescence (ACEL) shows great potential in lighting, display and intelligent skin. Herein, the ACEL properties of Pr3+-doped Ba0·85Ca0·15Ti0·90Zr0·10O3 ceramic its PDMS-based composite have been investigated. Intense red EL emission was obtained sample whereas blue Pr3+ observed for first time counterpart. The should be attributed to impact hot electrons driven by l...

Proteinase 3 is a serine protease found in neutrophil granules and on the extracellular neutrophil membrane (mPR3). mPR3 is a major antigen for anti-neutrophil cytoplasmic antibodies (PR3-ANCAs), autoantibodies causing fatal autoimmune diseases. In most individuals, a subpopulation of neutrophils also produce CD177, proposed to present additional PR3 on the surface, resulting in CD177neg/mPR3lo...

The treatment of the hydridorhodathiaboranes, [8,8,8-(H)(PR3)2-9-(Py)-nido-8,7-RhSB9H9], where PR3 = PPh3 (2), PMePh2 (3), PPh3 and PMe2Ph (4), or PMe3 and PPh3 (5), and [8,8,8-(H)(PMePh2)2-9-(PMePh2)-nido-8,7-RhSB9H9] (6), with TfOH affords [8,8,8-(H)(PR3)2-9-(Py)-nido-8,7-RhSB9H10](+) cations, where PR3 = PPh3 (12), PMePh2 (13), PPh3 and PMe2Ph (14), or PMe3 and PPh3 (15), and [8,8,8-(H)(PMeP...

In this work, we investigated synthesis, magnetic properties of silica coated metal ferrite, (CoFe2O4)/SiO2 and Manganese doped cobalt ferrite nanoparticles (MnxCo1-xFe2O4 with x= 0.02, 0.04 and 0.06)/SiO2 for possible biomedical application. All the ferrites nanoparticles were prepared by co-precipitation method using FeCl3.6H2O, CoCl2.6H2O and MnCl2.2H2O as precursors, and were silica coated ...

A 62-year-old man exhibiting nasal obstruction and glomerulonephritis with proteinase 3-antineutrophil cytoplasmic antibodies (PR3-ANCAs) was diagnosed with extranodal NK/T-cell lymphoma, nasal type (ENKL) with infiltration of neutrophils with apoptosis. Chemoradiotherapy reduced the tumor, improved the renal function, and decreased the PR3-ANCA levels. ANCA-positivity is observed in immunoinsu...

Anti-neutrophil cytoplasmic Abs (cANCAs) against conformational epitopes of proteinase 3 (PR3) are regarded as an important pathogenic marker in Wegener's granulomatosis (WG). Although the three-dimensional structure of PR3 is known, binding sites of mAbs and cANCAs have not been mapped to date. Competitive binding and biosensor experiments suggested the existence of four nonoverlapping areas o...