The Pvr4 resistance gene in pepper confers a complete resistance to the three pathotypes of potato virus Y (PVY) and to pepper mottle virus (PepMoV). In order to use this gene in a marker-assisted selection (MAS) program and to permit the pyramiding of several potyvirus resistance genes in the same cultivar, tightly linked amplified fragment length polymorphism (AFLP) markers were obtained by t...

Successful total RNA (totRNA) extraction is a prerequisite for a successful real-time PCR. In the present work we compared one manual and one automated totRNA extraction method for detection of Plum pox potyvirus (PPV) in leaves of different Prunus sp. using developed real-time RT-PCR assay. Advantages and disadvantages of compared methods are described in the view of sensitivity, reproducibili...

Lepidopteran types of podborer and homopteran aphid insects are damaging pests of chickpea. CryIAc toxins are established control agents for podborers but do not or only slightly affect homopteran (sucking) insects. However, certain plant lectins are known for their power of killing aphids. Bioassay experiments carried out in our laboratory with lectins isolated from garlic leaves have already ...

Genetic engineering projects often require control over when a protein is degraded. To this end, we use a fusion between a degron and an inactivating peptide that can be added to the N-terminus of a protein. When the corresponding protease is expressed, it cleaves the peptide and the protein is degraded. Three protease:cleavage site pairs from Potyvirus are shown to be orthogonal and active in ...

The main objective of this work was to investigate the ability of Aphis gossypii and Myzus persicae to transmit Cucumber mosaic virus (CMV) singly and mixed with two potyviruses (Papaya ringspot virus – type W, PRSV-W and Zucchini yellow mosaic virus, ZYMV), Pinto, Z.V.; Rezende, J.A.M.;Yuki,V.A.; Piedade, S.M.S.Ability ofAphis gossypii andMyzus persicae to transmitCucumbermosaic virus in singl...

Since 1991 epidemic dieback of the kelp Ecklonia radiata has been observed at the Leigh h4arine Reserve and other sites in northern New Zealand. As the dominant phaeophycean E. radiata is a very important component of the eastern coastal ecosystem, providing both food and refuge for many species. Examination of the E. radiata sap extracts by electron microscopy detected the presence of both str...

Effective population size (N(e)) is a key parameter for understanding evolutionary processes, but it is generally not considered in epidemiological studies or in studying infections of individual hosts. Whether N(e) has an effect on the onset of symptoms and viral accumulation in Tobacco etch virus (TEV) infection of Nicotiana tabacum plants is considered here. Using mixtures of TEV variants ca...