Five analogs of cyclic di-nucleotidic acid including c-di-GMP were synthesized and evaluated for their biological activities on Slr1143, a diguanylate cyclase of Synechocystis sp. Slr1143 was overexpressed from the recombinant plasmid which contained the gene of interest and subsequently purified by affinity chromatography. A new HPLC method capable of separating the compound and product peaks ...

A tripodal urea and tripodal thiourea with the same cyclic peptide core have been synthesised and their anion binding ability investigated. In CDCl(3), the tripodal urea self-associates whereas the thiourea does not. Neither compound shows self-association in the more polar 10% v/v DMSO-d(6)/CDCl(3). Both compounds bind strongly and selectively to sulfate ions in CDCl(3) and 10% v/v DMSO-d(6)/C...

Metabolite profiling has been established as a modern technology platform for the description of complex chemical matrices and compound identification in biological samples. Gas chromatography coupled with mass spectrometry (GC-MS) in particular is a fast and accurate method widely applied in diagnostics, functional genomics and for screening purposes. Following solvent extraction and derivatiz...

In the structure of the title compound, C(6)H(13)N(2)O(+)·C(2)H(3)O(2) (-)·H(2)O, the amide H atoms of the cations form centrosymmetric cyclic hydrogen-bonding associations incorporating two water mol-ecules [graph set R(4) (2)(8)], which are conjoint with cyclic water-bridged amide-amide associations [R(4) (4)(12)] and larger R(4) (4)(20) associations involving the water mol-ecule and the acet...

the studies performed about electrochemical oxidation 2-amino-4-methylphenol indicate that ortho-quinone yielded through oxidation of this chemical compound was unstable and it could be used as michael acceptor under solvent influences, carrier electrolyte, additives and could be transformed into the various compounds in the form of 1-4 addition michael reaction. based on this, in the paper fir...

The fast and selective determination of hydrogen peroxide (H2O2) is importance not only because strong interest in this widely applied analyte, but also the development enzymatic biosensors for glucose or other metabolites where sensor H2O2 can be used as transducer. Here, we report on an electrocatalytical amperometric detection H2O2. It a that consists gold electrode covered by self-assembled...

Proliferation of drug-resistant diseases raises the challenge of searching for new, more efficient antibiotics. Currently, some of the most effective antibiotics (i.e., Vancomycin and Daptomycin) are cyclic peptides produced by non-ribosomal biosynthetic pathways. The isolation and sequencing of cyclic peptide antibiotics, unlike the same activity with linear peptides, is time-consuming and err...

Abstract Rhodium(I)-catalyzed enantioselective cyclization of enynes through C(sp3)–H bond activation was investigated. It found that the having a tert-butyl moiety on alkene afforded spirocyclic compound (up to 92% ee), while an isopropyl or ethyl group gave cyclic diene 98% ee). Furthermore, intermolecular competition reaction using deuterium-labeled substrate revealed one key steps, high ene...

1,5,2,4-Dioxadithiepane-2,2,4,4-tetraoxide (cyclic-SoSo) is structurally a novel synthetic compound but may functionally act as an alkylating agent. The effects of cyclic-SoSo on DNA were studied in IMR-90 and VA-13 human embryo cells by means of DNA alkaline elution analysis. In contrast to a number of bifunctional alkylating agents, cyclic-SoSo produced no DNA-DNA interstrand cross-links in e...

We reevaluated the use of 32PO4 pulse-chases for analyzing mRNA decay rates in late-developing Dictyostelium cells. We found that completely effective PO4 chases could not be obtained in developing cells and that, as a consequence, the decay rates exhibited by some mRNAs were influenced by the rates at which they were transcribed. In developing cells disaggregated in the presence of cyclic AMP,...