Expressed sequence tags simple sequence repeats (EST-SSRs) are important sources for investigation of genetic diversity and molecular marker development. Similar to genomic SSRs, the EST-SSRs are useful markers for many applications in genetics and plant breeding such as genetic diversity analysis, molecular mapping and cross-transferability across related species and genera. In spite of low po...

Development of microsatellite markers has been an increasing trend in crop genetic studies because oftheir applicability in breeding programs. Here we report the development of inter simple sequencerepeat (SSRs) in pomegranate (Punica granatum L.) using an enrichment method that makes use of magneticbeads. Enriched genomic libraries with AG and ATG microsatellite motifs were c...

The present research aims to study the association and allelic diversity of linked microsatellite markers to grain quality QTLs of 84 exotic rice genotypes. To this end, 9 microsatellite markers (RM540, RM539, RM587, RM527, RM216, RM467, RM3188, RM246, RM5461) were used in which a total of 61 alleles were identified with a mean of 6 alleles per locus. The polymorphism information content (PIC) ...

Largemouth bass (Micropterus salmoides) is an economically important species in China. Contrary to its rapidly increasing yield during the last decades, domestic genetic diversity of largemouth has gradually declined. In this study, 37 single nucleotide polymorphism (SNP) markers were developed based on genotyping-by-sequencing data and characterized by genotyping 32 individuals using PCR–restr...

The morphological and molecular diversity of 101 peanut varieties from South China were analyzed to identify distinctness among these varieties. No significant difference was observed for six characteristics whereas a range 0.25–0.51 index 11 characteristics, with an average value 0.39. Molecular characterization 40 highly polymorphic simple sequence repeats (SSRs) generated total 167 alleles r...

Safflower is a self-pollinating oilseed crop and has been cultivated since antiquity in the Middle East. become minor crop, to increase its cultivation it necessary characterize introduce new germplasm. Thirty-eight safflower cultivars breeding lines from different countries have screened with 8 AFLP primer combinations assess genetic diversity relationships among these genotypes. primers produ...