Poly-ADP-ribosylation is a post-translational modification that regulates processes involved in genome stability. Breakdown of the poly(ADP-ribose) (PAR) polymer is catalysed by poly(ADP-ribose) glycohydrolase (PARG), whose endo-glycohydrolase activity generates PAR fragments. Here we present the crystal structure of PARG incorporating the PAR substrate. The two terminal ADP-ribose units of the...

Polymeric fibres with small radii (such as ≤125 nm) are delicate to handle and should be laid down on a solid substrate to obtain practical devices. However, placing these nanofibres on commonly used glass substrates prevents them from guiding light. In this study, we numerically and experimentally demonstrate that when the nanofibre is placed on a suitable dielectric multilayer, it supports a ...

−This paper presents the design, fabrication and characterization of a 32 by 32 electroplated micromirror array on a glass, a low cost substrate. Approaches taken in this work for the fabrication of micromachined mirror arrays include a line addressing scheme, a seamless array design for high fill factor, planarization techniques of polymeric interlayers, a high yield methodology for the remova...

Methods for fabricating poly(methyl methacrylate) microchips using a novel two-stage embossing technique and solvent welding to form microchannels in microfluidic devices are presented. The hot embossing method involves a two-stage process to create the final microchip design. In its simplest form, a mold made of aluminum is fabricated using CNC machining to create the desired microchannel desi...

Ultrafiltration reactors based on polymeric or ceramic membranes were shown to be suitable catalytic systems for fast enzymatic saccharification of cellulose, allowing the full recovery and reuse of enzymes. By pre-treating cellulose with the IL 1-butyl-3-methylimidazolium chloride, the suitability of this substrate for enzymatic saccharification in a reactor based on polymeric ultrafiltration ...

We consider the sticking of a fluid-immersed colloidal particle with a substrate coated by polymeric tethers, a model for soft, wet adhesion in many natural and artificial systems. Our theory accounts for the kinetics of binding, the elasticity of the tethers, and the hydrodynamics of fluid drainage between the colloid and the substrate, characterized by three dimensionless parameters: the rati...

New membrane types with well-controlled sub-nanostructures were developed from both polymeric and inorganic materials: carbon-based membranes, functionalized mesoporous oxide membranes and molecular sieving zeolite membranes. Regarding the carbon membranes, a novel carbon membrane with enhanced CO2 affinity was developed. The prepared membrane had a higher separation performance than that of th...

Cardiac failure is a quite severe condition that can result in life-threatening consequences. Cardiac tissue engineering is thought to be one of the most promising technologies to reconstruct damaged cardiac muscles and facilitate myocardial tissue regeneration. We report a new nanofiber bundle substrate for three-dimensional (3D) cardiac cell culture as a platform to investigate cell morpholog...

The impact of polymer removal by forming gas and vacuum annealing on the doping, strain, and morphology of chemical vapor deposited (CVD) and mechanically exfoliated (ME) graphene is investigated using Raman spectroscopy and atomic force microscopy (AFM). The behavior of graphene exposed and unexposed to polymer is compared. It is found that the well-known doping effect after forming gas anneal...

The double helix is a ubiquitous feature of RNA molecules and provides a target for nucleases involved in RNA maturation and decay. Escherichia coli ribonuclease III participates in maturation and decay pathways by site-specifically cleaving double-helical structures in cellular and viral RNAs. The site of cleavage can determine RNA functional activity and half-life and is specified in part by ...