results among the 181 anti-hcv ab negative patients, 13 (7.2%) were positive for hcv core ag and 11 (6%) had detectable hcv rna with a range of 40-336543 iu/ml by pcr. the two tests had a high measurement agreement (kappa=0.82, p<0.001). of the 13 patients with positive hcv core ag test results, 3 were negative for hcv rna. considering real time pcr for hcv rna as the gold standard for hcv infe...

conclusions the quite sensitive in-house taqman real time rt-pcr assay was able to detect and quantify all four main hcv genotypes prevailing around all geographical regions of iran. results the lower limit detection of this in-house hcv real-time rt-pcr was determined as 100 rna copies/ml. inter- and intra-assay coefficient of variation (cv) of this in-house hcv real-time rt-pcr ranged from 0....

background and aims: influenza virus is the most important cause of annual morbidities and mortalities worldwide with numerous antigenic drifts and shifts. inaccessibility to effective drugs and vaccines has made world health authorities to be interested in traditional medicine in order to prevent spread of the infectious agent. garlic is one of the most famous of all plants in human history. i...

background and objective: photobacterium damselae subsp. damselae is a marine pathogenic bacterium which causes disease in marine animals and human. this bacterium mostly found in coastal shallow seawater. so, the aim of this study was isolation and characterization of photobacterium damselae subsp. damselae from edible fish of persian gulf, bandar abbas. material and methods: totally 100 fish ...

background legionellaceae contains legionella genus with over 52 species and 64 serogroups. it is one of the most important causes of respiratory disease in human. more than 30% of hospital-acquired pneumonia is caused by legionella. ventilator-associated pneumonia (vap) is an infection acquired in hospital wards, particularly in intensive care unit (icu). this disease approximately affects 9% ...

results 34 (34%) of the renal transplant recipients and 26 (26%) of the control group were positive for anti-toxoplasma igg. in addition 18 (18%) of the renal transplant recipients and 4 (4%) of the control group were positive for anti-toxoplasma igm. for both values of the antibodies, differences were statistically significant (p < 0.025 and p < 0.001 respectively). the pcr results indicated t...

conclusions: the results showed that direct fusion of the recombinant plasmids containing the initial and ending parts of the target gene are simple and cost-effective techniques for increasing the length of the fragment and constructing ipc. results: the size of the pcr product was equal to the sum of the first and second fragments. sequencing confirmed the presence of the desire insert (ipc s...

background cervical cancer is the second most common cancer in women worldwide. recent studies show that human papillomavirus (hpv) dna is present in all cervical carcinomas and in some cervicitis cases, with some geographical variation in viral subtypes. therefore determination of the presence of hpv in the general population of each region can help reveal the role of these viruses in tumors. ...