Background: Interferon-gamma [IFN-γ) is the most important cytokine in immune system. This protein has been expressed bacterial cells. However, cloning not an easy task. We aimed to clone, express, and purify recombinant mouse IFN-γ overcome problems favor of commercial purposes. Materials Methods: To amplify gene product for cloning, we primarily designed two specific primers target gene. Foll...

Udang Vaname ( Litopenaeus vannamei) pada sebagian orang dapat memicu alergi bila dikonsumsi. Dengan pengolahan seperti proses perebusan diharapkan menurunkan sifat alergenitas protein udang tersebut. Adapun tujuan dari penelitian ini adalah untuk memperoleh karakterisasi terkait adanya perubahan profil bobot molekul (BM) alergen dan tingkat vaname selama perebusan. Metode yang digunakan di dal...

The effects of crude plasma and ethanol-extracted swamp eel at different concentrations (0, 0.25, 0.5, 0.75, 1, 1.5, 2 mg/g) as inhibitor protease in the quality tilapia (Oreochromis mossambicus) surimi were investigated. parameters analyzed gel strength, expressible moisture, whiteness, microstructure, protein solubility, hydrolysis pattern by sodium dodecyl sulfate-polyacrylamide electrophore...

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis is a technique that is used to separate proteins in a mixture for the purpose of identifying them individually. Proteins coated in SDS (which gives them a negative charge), are pulled through a thin gel by an electrical current. They separate on the gel based on size--the larger the protein, the slower it moves. Proteins can be stained w...

This study investigated calcium sulfate and chitosan on the textural modification microstructure of tofu made from lentils. The addition varying amounts (0–12 mM) (0–1.0%) into lentil milk could affect gel properties lentil-based tofu. properties, including hardness cohesiveness, significantly increased with 12 mM sulfate, exhibiting a slightly discontinuous network structure regular pore netwo...

Cell surfaces of aggregation, adherence, and hydrophilic variants of Streptococcus sanguis were compared with cell surfaces of the parent strain with regard to their protein and antigenic constituents. Cell surface molecules were released by digestion with mutanolysin. Extraction with sodium dodecyl sulfate (SDS) urea, lithium diiodosalicylate, and boiling water did not solubilize any material ...

Electrophoretic pattern of Bothrops alternatus, B.neuwiedi, B. ammodytoides, B. jararaca and B. jararacussu of Argentina by SDS-Polyacrylamide gel electrophoresis was carried out. Venoms used in this study came from animals (including juvenile) which live in different geographical zones. Special care was taken to not have cross-contamination of samples with blood and tissue fluids. All the snak...

Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of nuclear non-histone proteins extracted with 0.15 and 0.30 M NaCl from human normal lymphocytes or leukemic leukocytes indicated a lack of bands in the highermolecular-weight regions of the gels. However, the corre sponding fractions from phytohemagglutinin-stimulated lymphocytes and Burkitt lymphoma cells did contain highmolecul...