The reliability of an in vitro pyrogen test system based on proinflammatory cytokine release from human monocytic cells was assessed by comparison with a test system based on a human whole blood culture as well as with the conventional rabbit pyrogen test. The human cells used as the pyrogen indicator cells were newly selected by subcloning of a human monocytic cell line, Mono-Mac-6. The select...

Silicon nitride is a biocompatible material that is currently used as an interfacial surface between cells and large-scale integration devices incorporating ion-sensitive field-effect transistor technology. Here, we investigated whether a poly-L-lysine coated silicon nitride surface is suitable for the culture of PC12 cells, which are widely used as a model for neural differentiation, and we ch...

Gutta percha, the trans-isomer of polyisoprene, is being used for several technical applications due to its resistance to biological degradation. In the past, several attempts to isolate micro-organisms capable of degrading chemically pure poly(trans-1,4-isoprene) have failed. This is the first report on axenic cultures of bacteria capable of degrading gutta percha. From about 100 different hab...

Six strains of chloramphenicol (CM)-resistant endospore-forming bacteria, which can grow in the presence of 100 microgram/ml of CM, were isolated and identified as Bacillus badius. Mechanism of CM-resistance in one of the isolated strains, Bacillus badius 211, was investigated. No inactivation of CM was demonstrated when the strain was grown in nutrient broth containing 100 microgram/ml of CM, ...

Mouse myeloid leukemic Ml cells can be induced to differ entiate into mature macrophages and granulocytes by differ entiation-stimulating factor (D-factor) in conditioned medium of mouse peritoneal macrophages. Double-stranded RNA's, such as the copolymers of polyinosinic and polycytidylic acids and polyadenylic and polyuridylic acids, could not alone induce differentiation of the cells, but en...

Sugarcane (Saccharum officinarum L.) has wide range of economic importance due to its major and by-products. Seed multiplication of newly released varieties of sugarcane is one of the major constraints. Once a desired clone is identified, it usually takes 6-7 years to produce sufficient quality of improved seed material. This long duration causes a major bottleneck in breeding programmes. For t...

Stem cell therapy has emerged as the most vibrant area of research, due to capacity stem cells for self-renewal and differentiation into different types lines upon their culture. But lately, scientists become increasingly aware limitations conventional 2D culture media, several key drawbacks associated with this model, such immune response transplantation, animal pathogen contamination, complic...

Recent advances in nano- and micro-technology have made it possible to deliver surface-bound extracellular signaling cues to cultured neurons. In this study, we investigated the formation of neurites and axonal outgrowth using various types of polygonal micropatterns ('micropolygon arrays') on cell culture substrates and suggested a novel design principle of in vitro axon guidance. Ten differen...

objective: the development of combining mesenchymal stem cells (mscs) with surface modified three-dimensional (3d) biomaterial scaffold provides a desirable alternative for replacement of damaged and diseased tissue. nanofibrous scaffolds serve as suitable environment for cell attachment and proliferation due to their similarity to the physical dimension of the natural extracellular matrix. in ...