background: platanus species are widely cultured around the world and considered an important cause of allergic reactions. in the present study, we developed a sandwich elisa to quantify pla or 3 allergen in p. orientalis pollen extracts grown near high-traffic roads and compared it to pollen extracts collected from rural areas as control. methods: pollen samples were collected from three pollu...

pollen, honey, larvae, pupa and adult honey bees were collected from apiaries in taleghan, karadj region to assess the fungal infections.  several larvae were found in culture media of digestive system of adult bees and pollen through the experiment.  the larvae then were incubated to grow up and reach to the adult stage. morphological and systematic studies on samples of larvae and adults reve...

Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as 'barba-de-São-João', 'barba-de-bode', 'vassourinha branca', and 'mimosa'. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander's stain. The young inflorescences of twenty accessions were collected and fixed in a solutio...

pollen storage could facilitate the date palm hand pollination through saving time and space. the main concern is maintaining pollen viability, which is greatly cultivar dependent and is influenced by storage condition. in this study, to address this issue, the effects of cultivar, storage temperature, and time on pollen germination were investigated. a factorial experiment was laid out in a co...

Background: The Endothelial PAS domain-containing protein 1 (EPSA1) is the key transcriptional regulator of hypoxic response and Vascular Permeability Factor (VPF) is an important growth factor for vascular development and angiogenesis. OBJECTIVES: In the present study, the levels of the EPSA1 coding gene and VPF transcripts were evaluated during Larval development of Beluga, Huso huso. METHODS...

Methods Nasal secretions from 12 patients with AR to grass pollen and 12 healthy controls were collected. Two preweighed filter discs were placed bilaterally under direct visualization on the anterior third of the nasal septum. Five minutes later, they were removed and weighted again. After adding 2 ml 0,9% NaCl solution to the discs and centrifugation, the nasal secretions were stored in aliqu...

Vitamin A is considered an anti-infectious disease vitamin, and its deficiency is associated with severe infections such as in measles. In developing countries the low concentrations of vitamin A are a public health problem. The aim of this study is to describe serum vitamin A concentrations among children with visceral leishmaniasis (VL). Blood sample was collected from 22 children with VL, an...

air pollutants are reported to increase the specific ige response to allergens. to study allergen proteins and determine the effect of air pollutants on the allergenic potential of pollen extracts, the extracts were injected intraperitoneally and subcutaneously in guinea pigs. the results were analyzed by skin prick test (spt), elisa and sds-page followed by immunoblotting. the pollen extract o...