In this research, we propose a mass production method of gratings integrated with micro channels (GIMC) by means of an improved micro injection molding technique. The GIMC can be used together with a waveguide layer as a biochip in bio-medical assays. In the improved micro injection molding technique of this work, we fabricate an injection mold of gratings through the use of a nanoimprint and p...

Binding of Zn(II) by the ditopic ligands L1py, L2py and L1para, composed of a cyclam unit linked to the linear polyamines 1,4,8,11-tetraazaundecane (L1py and L2para) and 1,4,7-triazaheptane (L2py) via a 2,6-dimethylpyridinyl (L1py and L2py) or a 1,4-dimethylbenzyl spacer (L2para), has been analyzed by means of potentiometric and (1)H and (13)C NMR measurements. All ligands form stable mononucle...

BACKGROUND Colletotrichum truncatum is the most common pathogenic fungus associated with soybean anthracnose, a prevalent disease in Argentina. Pectinolytic enzymes are involved in the pathogenicity of a wide range of plant pathogenic fungi. OBJECTIVES To explore pectinolytic enzyme production in Argentinian Colletotrichum strains isolated from diseased soybean plants from different geographi...

OBJECTIVE To describe the phenotype of a patient with classical features of X-linked L1 syndrome associated with novel brain malformations. METHODS Diagnostic analysis included physical and dysmorphology examinations, MRI of the brain, and exome sequencing of the family trio. RESULTS We report a 2.5-year-old boy with developmental delay, dysmorphic facies, and adducted thumbs. MRI of the br...

A novel lysosomal phospholipase A(2) (LPLA2) with specificity toward phosphatidylethanolamine and phosphatidylcholine was previously purified and cloned. LPLA2 transfers sn-1 or sn-2 acyl groups of phospholipids to the C1 hydroxyl of the short-chain ceramide N-acetylsphingosine (NAS) under acidic conditions. The common features of lipophilic alcohols serving as acceptor molecules in the transac...

A 2 x 3 x 4 factorial field experiment was established to determine the interaction among a nematode, an insect, and three weed species on soybean in 1983-86. Low (nematicide treated) or high (untreated) population densities of the soybean cyst nematode (SCN), Heterodera glycines, and 0, 30, or 70% main stem girdling by the threecornered alfalfa hopper (TCAH), Spissistilus festinus, were combin...

BACKGROUND In the food industry, the use of pectinase preparations with high pectin esterase (PE) activity leads to the release of methanol, which is strictly regulated in food products. Herein, a pectin-degrading enzyme (PDE) complex exhibiting low PE activity of three Aspergillus sojae ATCC 20235 mutants (M3, DH56 and Guserbiot 2.230) was investigated. Production of exo-/endo-polygalacturonas...

We examined the concentration-dependent blocking effects of intracellular Mg2+ on L-type Ca2+ channels in cardiac myocytes using the whole cell patch-clamp technique. The increase of L-type Ca2+ channel current (I(Ca)) (due to relief of Mg2+ block) occurred in two temporal phases. The rapid phase (runup) transiently appeared early (<5 min) in dialysis of the low-Mg2+ solution; the slow phase be...

Given two sequences A = a 1 a 2 : : :a m and B = b 1 b 2 : : :b n , m n, over some alphabet , a common subsequence C = c 1 c 2 : : :c l of A and B is a sequence that can be obtained from both A and B by deleting zero or more (not necessarily adjacent) symbols. Finding a common subsequence of maximallength is called the Longest CommonSubsequence (LCS) Problem. Two new algorithms based on the wel...

Regulation of tissue-specific alpha-amylase (Amy) expression in Drosophila melanogaster was investigated with a newly developed method that detects the distribution of alpha-amylase allozymes in midguts of single adults or third-instar larvae. Trans regulation was found for alpha-amylase production in the posterior midgut (PMG) of adults, whereas cis regulation was demonstrated for the larval m...