In macroecology, the concept of r- and K-strategy has been widely applied, yet, there have limited studies on microbial life-history strategies in temperate grasslands using multiple sequencing approaches. Total phospholipid fatty acid (PLFA) analysis, high-throughput meta-genomic sequencing, GeoChip technologies were used to examine changes traits a chronosequence restored (1, 5, 10, 15, 25, 3...

Edible-fungal-based solid-state fermentation holds promise for sustainable food and biofuel production. Understanding the role of microbial communities in fungal substrates is crucial. Birch-based were treated with autoclaving (121 °C, at 2 bar) or hot air pasteurization (75–100 °C), followed by incubation without shiitake (Lentinula edodes) inoculum. Mycelial growth was monitored CO2 release b...

Lipid/DNA co-extraction from one sample is attractive in limiting biases associated with microbial community analysis from separate extractions. We sought to enhance established co-extraction methods and use high-throughput 16S rRNA sequencing to identify preferentially extracted taxa from co-extracted DNA. Co-extraction results in low DNA yields and distinct community structure changes.

Soil microorganisms and dissolved organic matter (DOM) play vital roles in nutrient cycling maintaining plant diversity. The aim of this study was to clarify the relationship between DOM component characteristics microbial community structure soil Larix principis-rupprechtii Mayr. plantations. We quantified responses stand age a plantation forest ecosystem using phospholipid fatty acid (PLFA) a...

A laboratory experiment was performed to examine the medium-term influence of three tetracycline antibiotics (chlortetracycline, CTC; tetracycline, TC and oxytetracycline, OTC) at different concentrations in four agricultural soils with similar pH soil organic content. After a 42-days incubation period, enzymes (?-glucosidase, urease, phosphomonoesterase) were estimated, as well phospholipid fa...

Although annual return of litter occurs in tea (Camellia sinensis L.) plantations, the level soil organic carbon (SOC) therein remains relatively low. The exact impacts pruned on and its association with SOC accumulation plantations remain unclear. In this study, we prepared 13C-labeled plant material incubated it soils collected at a plantation. Carbon dioxide (CO2) efflux sources were measure...

Abstract Purpose Urbanisation causes changes in land use, from natural or rural to urban, leading the sealing of soil and replacement vegetation by buildings, roads pavements. The process impacts properties services can lead negative consequences for microbial attributes processes soil. At present, information about community following is limited. As such, we investigated how physical chemical ...

It is well established that application of biochar to soils can promote soil fertility, which ultimately may enhance plant growth. While many mechanisms have been proposed explain this, one specific mechanism, the “microbial refugia hypothesis,” suggests provide physical protection for microbe from microfauna otherwise exert top-down control on microbial biomass and activity. We tested hypothes...

Abstract The transformation and turnover time of medium- to long-chain dicarboxylic acids (DCA) in soil is regulated by microbial uptake mineralization. However, the chain length n-alkyl lipids may have a remarkable influence on its utilization mineralization therefore formation stable organic carbon from e.g. leave- needle- root-derived matter during decomposition. To investigate their size de...