The stability of type 2 dengue virus to exposure to a variety of laboratory conditions was determined. Suckling mouse brain passage virus was adapted for growth in BHK-21 cells, and plaque assays were performed using a tragacanth gum overlay. A three- to fourfold increase in plaque size could be obtained if monolayers were subconfluent at time of inoculation. Incubation of virus for 24 h at 37 ...

BACKGROUND Despite continued preventive efforts, dental caries remains the most common disease of man. Organic acids produced by microorganisms in dental plaque play a crucial role for the development of carious lesions. During early stages of the pathogenetic process, repeated pH drops induce changes in microbial composition and favour the establishment of an increasingly acidogenic and acidur...

objective: mechanical plaque removal namely tooth brushing is the most commonly used oral hygiene technique. efficacy of tooth brushing is influenced by the design and shape of toothbrush. this study aimed to compare the efficacy of two common iranian toothbrushes with a foreign made one in plaque removal.   methods: this clinical trial was conducted on 30 female dental students in babol univer...

Plaque 'calcium-fluoride-like' (CaF(2)-like) and fluoride deposits held by biological/bacterial calcium fluoride (Ca-F) bonds appear to be the source of cariostatic concentrations of fluoride in plaque fluid. The aim of this study was to quantify the amounts of plaque fluoride held in these reservoirs after a sodium fluoride rinse. 30 and 60 min after a 228 microg/g fluoride rinse, plaque sampl...

background: chewing-gum may serve as an effective oral hygiene device when brushing may not be possible. the aim of the present study was to evaluate the effect of chewing sugar-free gum twice a day after meals in addition to tooth brushing on dental plaque and interdental debris.     methods: twenty four (12 males and 12 females aged 20-21 years) healthy third-year dental students participated...

BACKGROUND Many human microbial infectious diseases including dental caries are polymicrobial in nature. How these complex multi-species communities evolve from a healthy to a diseased state is not well understood. Although many health- or disease-associated oral bacteria have been characterized in vitro, their physiology within the complex oral microbiome is difficult to determine with current...

Background. Topical application of manuka honey is effective in the treatment of burns and soft-tissue infections. The aim of this study was to assess the antibacterial activity of manuka honey against plaque-associated bacteria in vitro in order to evaluate the potential application as an adjunct to periodontal treatment. Materials and Methods. The minimum bacteriostatic and bactericidal conce...

Background: Numerous studies have demonstrated the decline in Streptococcus mutans level following consumption of Probiotic. However, most them tested salivary levels microbes. Aim: The aim present study was to assess effect probiotic curd on pH and colony count Mutans dental plaque. Subjects methods: This randomized control trial conducted 20 healthy children 6-12 years age. subjects were rand...

To study some biological and molecular properties of nine DENV-2 strains isolated during the 1981 Cuban epidemic, temperature sensitivity, viral plaque size, the kinetic of virus replication in newborn mice inoculated by intracerebral route, the influence of pH medium on virus-cell attachment phase and the restriction enzyme pattern were studied. Strains were classified in two patterns accordin...