نتایج جستجو برای: pkm2

تعداد نتایج: 844  

2017
Yiming Yang Ke Wu Yulin Liu Liang Shi Kaixiong Tao Guobin Wang

BACKGROUNDS Numerous studies have reported that aberrant pyruvate kinase M2 isoform (PKM2) expressed in cancer, indicating that PKM2 plays a critical role in tumor initiation and progression. Nevertheless, its prognostic value in breast cancer tumor is yet contentious. Therefore, we performed this meta-analysis to evaluate the prognostic significance of PKM2 in breast cancer. METHODS Eligible...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2012
Jiangbin Ye Anthony Mancuso Xuemei Tong Patrick S Ward Jing Fan Joshua D Rabinowitz Craig B Thompson

Despite the fact that most cancer cells display high glycolytic activity, cancer cells selectively express the less active M2 isoform of pyruvate kinase (PKM2). Here we demonstrate that PKM2 expression makes a critical regulatory contribution to the serine synthetic pathway. In the absence of serine, an allosteric activator of PKM2, glycolytic efflux to lactate is significantly reduced in PKM2-...

Journal: :Molecular cell 2015
Sophia Y Lunt Vinayak Muralidhar Aaron M Hosios William J Israelsen Dan Y Gui Lauren Newhouse Martin Ogrodzinski Vivian Hecht Kali Xu Paula N Marín Acevedo Daniel P Hollern Gary Bellinger Talya L Dayton Stefan Christen Ilaria Elia Anh T Dinh Gregory Stephanopoulos Scott R Manalis Michael B Yaffe Eran R Andrechek Sarah-Maria Fendt Matthew G Vander Heiden

Metabolic regulation influences cell proliferation. The influence of pyruvate kinase isoforms on tumor cells has been extensively studied, but whether PKM2 is required for normal cell proliferation is unknown. We examine how PKM2 deletion affects proliferation and metabolism in nontransformed, nonimmortalized PKM2-expressing primary cells. We find that deletion of PKM2 in primary cells results ...

Journal: :Acta biochimica et biophysica Sinica 2013
Songfang Wu Huangying Le

Cancer cells have distinct metabolism that highly depends on glycolysis instead of mitochondrial oxidative phosphorylation alone, known as aerobic glycolysis. Pyruvate kinase (PK), which catalyzes the final step of glycolysis, has emerged as a potential regulator of this metabolic phenotype. Expression of PK type M2 (PKM2) is increased and facilitates lactate production in cancer cells, which d...

2015
Kohei Taniguchi Yuko Ito Nobuhiko Sugito Minami Kumazaki Haruka Shinohara Nami Yamada Yoshihito Nakagawa Tarou Sugiyama Manabu Futamura Yoshinori Otsuki Kazuhiro Yoshida Kazuhisa Uchiyama Yukihiro Akao

The Warburg effect is a well-known feature of cancer cells. However, its' functional significance hasn't been elucidated yet. Pyruvate kinase muscle (PKM), which is a rate-limiting glycolytic enzyme, has 2 isoforms, PKM1 and PKM2. It has been reported that PKM2 is a tumor-specific isoform and promotes the Warburg effect. Also, it has been thought that tumor cells switch their PKM isoform from P...

Journal: :International journal of clinical and experimental pathology 2015
Suijun Wang Zhen Yang Ying Gao Quanzhong Li Yong Su Yanfang Wang Yun Zhang Hua Man Hongxia Liu

Failure of pancreatic β-cells is closely associated with type 2 diabetes mellitus (T2DM), an intractable disease affecting numerous patients. Pyruvate kinase, muscle isoform 2 (PKM2) is a potential modulator of insulin secretion in β-cells. This study aims at revealing roles and possible mechanisms of PKM2 in pancreatic β-cells. Mouse pancreatic β-cell line NIT-1 was used for high glucose treat...

2015
Cheng Zhan Li Yan Lin Wang Jun Ma Wei Jiang Yongxing Zhang Yu Shi Qun Wang

Muscle type of pyruvate kinase (PKM) is one of the key mediators of the Warburg effect and tumor metabolism. Due to alternative splicing, there are at least 12 known isoforms of the PKM gene, of which PKM1 and PKM2 are two major isoforms with only a 23 amino acid sequenced difference but quite different characteristics and functions. It was previously thought the isoform switch from PKM1 to PKM...

Journal: :Biochemical Journal 2021

Redox regulation of proteins via cysteine residue oxidation is involved in the control various cellular signal pathways. Pyruvate kinase M2 (PKM2), a rate-limiting enzyme glycolysis, critical for metabolic shift from glycolysis to pentose phosphate pathway under oxidative stress cancer cell growth. The PKM2 tetramer required optimal pyruvate (PK) activity, whereas inhibition inter-subunit inter...

2018
Jian Lu Min Chen Sumeng Gao Jigang Yuan Zhu Zhu Xiaoping Zou

The 'Warburg effect' is considered a vital hallmark of cancer cells, characterized by an altered metabolism, in which cells rely on aerobic glycolysis. As a key enzyme of aerobic glycolysis, pyruvate kinase M2 (PKM2) serves a crucial role in tumorigenesis. Accumulating studies have indicated that PKM2 is a potential target for cancer therapy. The aim of the present study was to assess the antic...

2014
Qi Li Xue Liu Yu Yin Ji-Tai Zheng Cheng-Fei Jiang Jing Wang Hua Shen Chong-Yong Li Min Wang Ling-Zhi Liu Bing-Hua Jiang

Although insulin is known to regulate glucose metabolism and closely associate with liver cancer, the molecular mechanisms still remain to be elucidated. In this study, we attempt to understand the mechanism of insulin in promotion of liver cancer metabolism. We found that insulin increased pyruvate kinase M2 (PKM2) expression through reactive oxygen species (ROS) for regulating glucose consump...

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