Novel insertion sequence (IS)-like elements were isolated and characterized from phytoplasma strains in the aster yellows (AY) group (16SrI). The IS-like elements were cloned from phytoplasma strains AY1 and NJAY or PCR-amplified from 15 additional strains representing nine subgroups in the AY group using primers based on sequences of the putative transposases (Tpases). All IS-like elements con...

Little leaf and yellowing symptoms associated with phytoplasma disease were observed in Pyracantha angustifolia Turkey. Amplification (through nested PCR) virtual restriction fragment length polymorphism (V-RFLP) confirmed the presence of symptomatic plants phylogeny based on R16F2n/R2 sequences P. was established sequence analysis showed that isolate had 98.7% similarity to ‘Candidatus Phytopl...

Gejala kuning mirip seperti infeksi fitoplasma ditemukan pada pertanaman wortel di Jawa Barat. Penelitian bertujuan mengidentifikasi tanaman dan serangga wereng yang berasosiasi dengan gejala Bogor Bandung. Wereng terdiri atas 5 spesies daun (Hemiptera: Cicadellidae), yaitu Balclutha incisa (Matsumura), Orosius argentatus (Evans), Cicadulina bipunctata Empoascanara indica (Datta), Exitianus ind...

Sisal purple leafroll disease (SPLD) is a destructive affecting sisal in China, however, its etiology remains unknown. This characterized by margins and rolling yellowing of the leaves. Recently, phytoplasma was found to be present SPLD-affected according PCR assay. To further verify presence phytoplasma, determine association with SPLD identify possible pathogenic infecting sisal, 80 sym...

Apple proliferation (AP) and pear decline (PD) are the most severe diseases in pome fruit growing areas. AP-infected trees show typical symptoms such as witches’ broom, enlarged stipules, tasteless, and dwarf fruits. PD-infected pears show a progressive weakening, reduced terminal growth, smaller fruits, and die within weeks (quick decline) or years (slow decline). The diseases are caused by th...

Phytoplasmas are insect vectored mollicutes responsible for disease in many economically important crops. Determining which insect species are vectors of a given phytoplasma is important for managing disease but is methodologically challenging because disease-free plants need to be exposed to large numbers of insects, often over many months. A relatively new method to detect likely transmission...

Witches' broom disease of lime (WBDL) is a serious phytoplasma disease of acid lime in Oman, the UAE and Iran. Despite efforts to study it, no systemic study attempted to characterize the relationship among the associated phytoplasma, 'Candidatus Phytoplasma aurantifolia', from the three countries. This study utilized sequences of the 16S rRNA, imp and secA genes to characterize 57 strains coll...

BACKGROUND Phytoplasmas are bacterial phytopathogens responsible for significant losses in agricultural production worldwide. Several molecular markers are available for identification of groups or strains of phytoplasmas. However, they often cannot be used for identification of phytoplasmas from different groups simultaneously or are too long for routine diagnostics. DNA barcoding recently eme...

During 2021 surveys, greengage witches’-broom (GWB) and decline (GD) diseases were respectively observed in Fars Yazd provinces of Iran. Presence phytoplasma the symptomatic plants was confirmed by PCR amplifications 16S rRNA gene. Sequence analyses showed affiliation GWB GD phytoplasmas to‘Candidatus Phytoplasma phoenicium’ (16SrIX-B) and‘Ca. P. aurantifolia’ (16SrII-C) related strains, respec...

Pear decline is a very important phytoplasma disease that causes considerable quantitative and qualitative losses to this fruit crop. Due to economical importance of pear in Isfahan province, Iran, and the difficulty to determine the occurrence of the disease simply based on symptoms in orchards, a detection method for the phytoplasma causing disease in pears in the region was developed. Since ...