A procedure for the determination of serum high-density lipoprotein choline-containing phospholipids is described. The choline-containing phospholipids represent 91-97% of the total serum and high-density lipoprotein phospholipids, which have previously been determined in relation to liver disease, neoplastic disorders, diabetes mellitus and atherosclerosis. The enzymatic assay is quick, simple...

This study was carried out to determine the major phospholipids in selected dairy products (Beta Serum, Procream and Phospholipids-Rich Dairy products), evaluate accuracy of developed fat extraction method for liquid samples compare different obtained using HPLC-UVvis 31P-NMR methods. It found that can be used estimate lipid content were still a bit satisfactory. Using method, it sphingomyelin ...

A simple and reproducible method for altering the acyl chain composition of Chinese hamster ovary cell phospholipids is described. By supplementation of the medium with 5% delipidized serum and 12 pg/ml of various fatty acids, extensive incorporation of the added fatty acid into membrane phospholipids was obtained. No significant changes in the phospholipid head group composition or the sterol/...

We have developed an improved thin-layer chromatographic method for separation of lung phospholipids. Individual phospholipids are completely separated in the first dimension. All phospholipids, except phosphatidylcholine, are then removed. The phosphatidylcholine-containing area is reacted with osmium tetroxide and saturated phosphatidylcholine species are separated from the unsaturated oxidat...

1. The analysis, by silica-gel chromatography, of phospholipids from a human-plasma fraction is described. The fraction was derived from whole blood which had been incubated with sodium [Me-14C]acetate for 6-5 hr. The #-lipoproteins had been removed by precipitation with dextran sulphate. 2. All the phospholipids isolated were labelled. 3. The fatty acids of the isolated phospholipids were anal...

The regulated movement of glucose across mammalian cell membranes is mediated by facilitative glucose transporters (GLUTs) embedded in lipid bilayers. Despite the known importance of phospholipids in regulating protein structure and activity, the lipid-induced effects on the GLUTs remain poorly understood. We systematically examined the effects of physiologically relevant phospholipids on gluco...

APS is an auto immune disease in which auto antibodies are detected against cell membrane phospholipids and is clinically characterised mainly by thrombocytopenia, thrombosis, neurologic and dermatologic manifestation and recurrent fetal loss. More recently it is found that these antibodies react with specific plasma proteins associated with phospholipids (e.g. β2 glycoprotein1 β2gp1) rather th...

Lipid content and capacity to incorporate in vitro palmitate-1-14C and linoleate-114C into lipids was investigated in fresh and stored (4#{176}C)human platelets. Cholesterol and phospholipids decreased 30% during storage for 6 days. Molar ratio of cholesterol to phospholipids and percentage distribution of individual phospholipids were similar in fresh and stored platelets. Palmitate bound to a...

High-field 31P nuclear magnetic resonance spectroscopy was used to quantitate phospholipids in mixtures in organic solvents. The sample is dissolved in chloroform-methanol and analyzed at 161.7 MHz with decoupling of the protons. Signals were identified using authentic compounds, and their relative distribution was measured in mole percent. The method has good accuracy and reproducibility, and ...

Work by Marinetti, Erbland & Stotz (1958) on pig-heart-cell fractions and by Macfarlane, Gray & Wheeldon (1960) on rat-liver-cell fractions have indicated that the mitochondrial and microsomal phospholipids may differ significantly in composition. In view of the results obtained by Collins (1960) and by Collins & Shotlander (1961), showing the widespread occurrence of complex amino-phospholipid...