Erythrocyte ghosts have been found to be peculiarly useful for the study of the metabolism of nucleosides (1, 2). Preparations made by osmotic hemolysis were found to be unable to metabolize glucose. Adenosine, however, caused a disappearance of inorganic phosphate from ghost plasma suspensions with an equivalent production of organic acid-soluble ester phosphate. There was a simultaneous disap...

An efficient synthesis of a phosphinate analogue of the anti-tumour phosphate di-ester perifosine is described (6 steps and 50% overall yield). The two phosphorus-carbon bonds in the perifosine analogue were prepared by sequential double radical hydrophosphinylation processes. This is the first example of a phosphinate analogue of perifosine, designed to be resistant to hydrolysis by phospholip...

A cell free system prepared from etiolated cucumber (Cucumis sativus, L) in tris-sucrose buffer is able to incorporate delta-aminolevulinic acid-4- (14)C into the two components of protochlorophyll: protochlorophyllide and protochlorophyllide ester. The activity is associated with the etioplasts. Optimal incorporation is obtained at pH 7.7. For the formation of protochlorphyllide ester, oxygen,...

Essential hypertensive patients show a reduced nitric oxide availability secondary to oxidative stress generation in peripheral microcirculation. Cyclooxygenase (COX) contributes to reduce nitric oxide availability. We assessed the possible vascular sources of oxidative stress, including COX-1, COX-2, and nicotinamide adenine dinucleotide phosphate oxidase, as determinants of endothelial dysfun...

Lack of long-time stability dsDNA-based supramolecular assemblies is an important issue that hinders their applications. In this work, 20 base pairs long dsDNA fragments [(dCdG)20-65 %] composed 65 % dCdG and 35 dAdT nucleotides were tethered via a thiol to the surface gold electrode. The self-assembled (dCdG)20-65 monolayer was immersed in solutions containing ectoine, compatible solute. Elect...

Particles of certain rat tissue preparations contain a phosphate ester hydrolase that destroys carbamyl phosphate. This activity is similar to that of the alkaline phosphatases. Both have the same molar activities, pH curves, and particulate localization. Both are activated when solubilized with 1butanol and are inhibited by adenine nucleotides and inorganic phosphate. Their reactions are not a...

The title mol-ecule, C8H12NO5PS2, exhibits a crystallographic mirror plane that is perpendicular to the ring and bis-ects the sulfamoyl and thio-phosphate ester groups. In the crystal, mol-ecules are linked by N-H⋯O hydrogen-bonding inter-actions reminiscent of carb-oxy-lic acid hydrogen bonding pairs, forming chains parallel to the b-axis direction.

Oseltamivir phosphate is an antiviral drug used in the treatment and prophylaxis of both influenza virus A and influenza virus B. Oseltamivir phosphate is an ethyl ester pro-drug that is rapidly and extensively metabolized by esterases in the gastrointestinal tract and liver to its active form, oseltamivir carboxylate. There are some analytical methods in the literature for the analysis of both...