The stereochemically constrained chemotactic peptide analogs, formylmethionyl-alpha-aminoisobutyryl-phenylalanine (formyl-Met-Aib-Phe-OH) and formylmethionylcycloleucinylphenylalanine (formyl-Met-Cyl-Phe-OH) are highly effective in inducing lysosomal enzyme release from rabbit neutrophils. NMR studies of the Aib2 analog in (CD3)2SO favor a folded conformation in which the Phe NH group is inacce...

We retrospectively examined the relationship between blood biomarkers, in particular historical mean phenylalanine to tyrosine (Phe:Tyr) ratio, and cerebral glucose metabolism. hypothesized that Phe:Tyr ratio would be more predictive of metabolism than (Phe) level alone. performed a retrospective case series analysis involving 11 adult classical phenylketonuria/hyperphenylalaninemia patients un...

An innovative double network hydrogel based on poly(vinyl-alcohol) (PVA) cross-linked with glutaraldehyde (GTA) was obtained by the addition of self-assembling phenylalanine (Phe) derivatives aim to achieve improved mechanical-elastic properties exploitable produce 3D dosimeters. The ability in fibrous structures Phe (FmocPhe-OH, A; FmocPhe-Phe-OMe, P) even within PVA gel proved AFM and SEM ima...

Antibodies with specificity to a single species of tRNA were elicited in a goat by immunization with a glutaraldehyde conjugate of yeast phenylalanine transfer RNA with bovine gamma globulin. The specificity of the antibodies was studied by a radioimmunoassay measuring the direct binding of [(3)H]tRNA(Phe) or the inhibition of the binding. The antibodies formed are predominantly directed toward...

In this paper, methods of sample preparation for potentiometric measurement of phenylalanine are presented. Basing on the spectrophotometric measurements of phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe, 0,4 mM NAD, 2U L-PheDH) were determined. Then, the absorption spectrum of the reaction product, NADH, was monitored (maximum peak at 340 nm). The results ...

Pt(C/N)(phe)(1, C/N = 2-(2'-thienyl)pyridine, phe = phenylalanine) shows a high binding affinity (ca. 10(6) dm(3) mol(-1)) and selectivity towards human serum albumin (HSA) and such binding is accompanied by an enhancement of photoluminescence at 562 nm; both the protein binding affinity and cytotoxicities of [Pt(C/N)(phe)(1), Pt(C/N)(trp)(2, trp = tryptophan) and Pt(C/N)(gly)(3, gly = glycine)...

its purification a benzoylated diethylaminoethyl cellulose column (7), after which we passed the preacylated tRNAsi^c: over an RPC-II column. This last column is described in Chart 1. The Chromatographie profile of the phenylalanine acceptor activity presented 3 distinct peaks. Those indicated as Phe 1 and Phe 2 were also normally present in tRNAs^a 0); Phe X is a new tRNA that we first observe...

Based on experimental data from fermentation runs, as well as from L-phenylalanine (l-Phe) separation studies, a simple model is presented that describes the total ISPR approach for on-line L-Phe separation. While fermentation process modeling via a macrokinetic model revealed an L-Phe inhibition constant of 20 +/- 1.35 g/L using recombinant E. coli cells, the reactive-extraction process modeli...

We describe a term born boy of non-consanguineous Swiss parents with tetrahydrobiopterine (BH4)-responsive Phenylketonuria (PKU) and Hirschsprung disease with unusual neonatal presentation. The child presented with floppiness, irritability, recurrent bilious vomiting and failure to pass meconium until 32 hours after birth, resulting in the clinical suspicion of an intoxication-type metabolic di...