Phagocytosis of extracellular collagen by fibroblasts appears to be the principal pathway of collagen degradation in the physiological turnover of connective tissues. To study the mechanism of collagen phagocytosis, subconfluent gingival fibroblasts were serum-starved and incubated for up to 16 h with collagen-coated fluorescent latex beads. Internalization of beads was measured either by flow ...

Background Inflammation is one of the critical components in development neurodegenerative diseases. Microglia resident macrophages central nervous system. In AD brain, microglial cells are found close association with amyloid beta deposits. Histological examination brains as well cell culture studies have shown that interaction microglia fibrillar leads to their phenotypic activation. It incre...

When expressed on the surface of cells, CD47 inhibits phagocytosis of these cells by phagocytes. Most human cancers overexpress CD47, and antibodies to CD47 have shown a remarkable ability to clear a range of cancers in animal models. However, the mechanism by which these antibodies cause cancer cell death is unclear. We find that CD47 is expressed on the surface of three B-cell lines from huma...

In chronic myelogenous leukemia (CML), the mature granulocytes originate from a stem cell line harboring an abnormal chromosome, therefore it is possible that metabolic-functional abnormalities occur in the morphologically mature cells. In the present study, the phagocytic activity including intracellular killing, nitro blue tetrazolium (NBT) reduction, and phagocytosis were studied in 37 CML p...

background: enhancement of the immune system seems to be the most promising method of preventing fish diseases and increasing growth rate. objectives: the purpose of the present study was to investigate the influence of dietary administration of zataria multiflora boiss and menthapulegium l extracts on phagocytosis, lysozyme, respiratory burst and total white and red blood cells (wbc/rbc) of ra...

this study was conducted to investigate the effects of curcuma zedoaria and zingiber zerumbet on non-specific immune responses of grouper (epinephelus coioides). fish were fed an experimental diet containing 0, 0.5, 1.0, 2.5 g/kg of c. zedoaria and z. zerumbet mixed diets twice daily for two weeks. non-specific immune parameters such as respiratory burst activity, reactive oxygen species, phago...

Phagocytosis is a critical host defense mechanism of neutrophils. In this study, membrane protein changes occurring during phagocytosis were studied in human neutrophils using surface radiolabeling before or after phagocytosis of various target particles. Cells were labeled at the cell surface using lactoperoxidase-catalyzed iodination or neuraminidase-galactose oxidase-NaB3H4, galactose oxidas...

The effect of colchicine on the uptake of oxygen by human leukocytes during phagocytosis of live streptococci or of killed staphylococci was compared with the effect of colchicine on phagocytosis per se, measured in a sensitive bacterial system. The increase in oxygen consumption that normally accompanies phagocytosis was consistently diminished in leukocytes incubated with colchicine in concen...

Our previous studies showed that urinary Tamm-Horsfall glycoprotein (THP) potently enhanced polymorphonuclear neutrophil (PMN) phagocytosis. However, the domain structure(s), signaling pathway and the intracellular events responsible for THP-enhanced PMN phagocytosis remain to be elucidated. THP was purified from normal human urine. The human promyelocytic leukemia cell line HL-60 was induced t...