Restriction fragment length polymorphism (RFLP) analysis with probes derived from the insertion element IS6110, the direct repeat sequence, and the polymorphic GC-rich sequence (PGRS) and a PCR-based typing method called spacer oligonucleotide typing (spoligotyping) were used to strain type Mycobacterium bovis isolates from the Republic of Ireland. Results were assessed for 452 isolates which w...

The use of IS6110 as a marker for molecular epidemiological studies is limited when a Mycobacterium tuberculosis isolate has five or fewer copies of IS6110. Restriction fragment length polymorphism analysis with a highly polymorphic GC-rich repetitive sequence located in the plasmid pTBN12 (PGRS RFLP) and spoligotyping (based on the polymorphism of the DR region) are two frequently used seconda...

Strawberry is a valuable, nutritious, and economically important fruit all over the world including Bangladesh. Therefore, there is a demand to develop a suitable variety of strawberry. For this purpose, leaf explants from in vitro grown strawberry plantlets were cultured onto MS medium supplemented with different concentrations and combinations of 2,4-D, NAA and BA for callus induction. The mo...

Plant growth regulators (PGRs) play a key role in regulating physiological and morphological development of plant species. Application of these PGRs can improve preand post-harvest quality and quantity of ornamental flowers and lessen the harmful effects of injury incurred at harvesting time. The objective of this study was to determine the appropriate spraying solution for improving preand pos...

Submit Manuscript | http://medcraveonline.com Abbreviations: HPLC: High Pressure Liquid Chromatography; DAD: Diode Array Detector; RSD: Relative Standard Deviation; RE: Relative Error; CPF: Cupressuflavone; AMF: Amentoflavone; CNC: Cupressus from North Coast, Egypt; CFU: Cupressus from Future University, Egypt; MS: Murashige and Skoog medium; PGRS: Plant Growth Regulators; NAA: β-Naphthalene Ac...

Wheat is the cereal most susceptible to lodging, particularly during flowering period and at early ripening stage. The use of plant growth retardants (PGRs) especially recommended when intensive nitrogen (N) fertilisation applied, which increases susceptibility plants lodging. This paper presents results tests into effects PGRs (PGR0—control; PGR1—chlormequat chloride (CCC) + trinexapac-ethyl (...

Lodging is one of the main factors causing yield loss maize under high-density planting conditions. Root lodging as an important type has received little attention. Plant growth regulators (PGRs) and nitrogen fertilizer can coordinate relationship between root yield. This two-year field experiment was conducted with two levels N225 (225 kg ha−1) N300 (300 at a high density (90,000 plants during...

this review article investigates a comprehensive description of the factors, methods, strategies, approaches and prerequisites influencing in vitro micropaopagation and the amount of their use across the world. the findings presented here are the results of the study of several hundred papers published all over the world. tissue culture techniques are routinely used for micropropagation. enhanc...

Six compounds (IBA, chlorogenic acid, cytokinine, GA3, alar B-9 and maleic hydrazide) belonging to four different categories of plant growth hormones were used to study their effect on carbohydrate content in L. erysimi. The second instar nymphs (48 hr old) were given both dipping and leaf surface treatment with 1024 ppm concentration of compounds for two time intervals i.e. 48 and 96 hr. The c...