Bioremediation is an effective measure in dealing with such contamination, particularly those from petroleum hydrocarbon sources. The effect of soil amendments on diesel fuel degradation in soil was studied. Diesel fuel was introduced into the soil at the concentration of 5 % (w/w) and mixed with three different organic wastes tea leaf, soy cake, and potato skin, for a period of 3 months. Withi...

Oil contaminated soil, sea water and sediment samples were collected from old oil jetty of Paradip port, Orissa. These samples were screened for bacterial oil degradation using 0.5% diesel in Bushnell-Hass Mineral Salt medium. Samples were incubated separately in shaking orbital incubator at 37 0 C at 125rpm up to 15days. The level of petroleum hydrocarbon degradation was determined by gravimet...

Current bioremediation techniques for petroleum-contaminated soils are designed to remove contaminants as quickly and efficiently as possible, but not necessarily with postremediation soil biological quality as a primary objective. To test a simple postbioremediation technique, we added earthworms (Eisenia fetida) or wheat (Triticum aestivum) straw to petroleum land-farm soil and measured biolo...

Evidence for on site biodegradation may be difficult to provide at heterogeneous sites without additional experiments in controlled laboratory conditions. In this study, microbial activities measured as CO2 and CH4 production were compared in situ, in intact soil cores and in bottle microcosms containing sieved soils. In addition, biodegradation rates were determined by measuring the decrease i...

Bacterial community dynamics were investigated in a land treatment unit (LTU) established at a site contaminated with highly weathered petroleum hydrocarbons in the C(10) to C(32) range. The treatment plot, 3,000 cubic yards of soil, was supplemented with nutrients and monitored weekly for total petroleum hydrocarbons (TPH), soil water content, nutrient levels, and aerobic heterotrophic bacteri...

Petroleum hydrocarbons are contaminants of particular significance. With production rates of 13.26 million cubic meters of crude oil per day, their potential as soil and water contaminant is apparent. The most commonly used analytic method for assessing Total Petroleum Hydrocarbons (TPH) in soil samples, is based on extraction with 1,1,2-Trichlorotrifluoroethane (Freon 113) , a substance prohib...

Bioelectroremediation (BER) uses electrical current to stimulate catabolism of environmental pollutants, such as petroleum spills. However, applying soil may have adverse effects on the microorganisms involved in degradation. We identify and evaluate BER capabilities a strain Aspergillus niger obtained from petroleum-contaminated soil. Spores this immobilized alginate spheres (2 g) were mixed w...

Landfarm soil is used to bioremediate oil wastes from petrochemical industries. We developed a simplified protocol for microbial DNA extraction of tropical landfarm soil using only direct lysis of macerated material. Two samples of tropical landfarm soil from a Brazilian refinery were analyzed by this protocol (one consisted of crude oil-contaminated soil; the other was continuously enriched fo...

Genes that encode for enzymes that can degrade petroleum hydrocarbons (PHs) are critical for the ability of microorganisms to bioremediate soils contaminated with PHs. Distributions of two petroleum-degrading genes AlkB and Nah in soils collected from three zones of the Dagang Oilfield, Tianjin, China were investigated. Numbers of copies of AlkB ranged between 9.1 × 10(5) and 1.9 × 10(7) copies...