Treatment of melon hypocotyls or petioles with an elicitor from Colletotrichum lagenarium, a fungal pathogen of melons, causes an initial transitory inhibition of protein synthesis and, after 18 hours, induces the synthesis of a plant cell wall hydroxyproline-rich glycoprotein (HRGP). Microgram amounts of elicitor are sufficient for maximum elicitation of HRGP when the elicitor is injected into...

The first step in gibberellin biosynthesis is catalyzed by copalyl diphosphate synthase (CPS) and ent-kaurene synthase. We have cloned from pumpkin (Cucurbita maxima L.) two cDNAs, CmCPS1 and CmCPS2, that each encode a CPS. Both recombinant fusion CmCPS proteins were active in vitro. CPS are translocated into plastids and processed by cleavage of transit peptides. For CmCPS1 and CmCPS2, the put...

Plant petioles and stems are hierarchical cellular structures, displaying structural features defined at multiple length scales. The current work focuses on the multi-scale modelling of plant tissue, considering two orders of structural hierarchy, cell wall and tissue. The stiffness of plant tissue is largely governed by the geometry of the tissue cells, the composition of the cell wall and the...

Translocation of a green fluorescent protein (GFP)-tagged Dickeya sp. from stems or from leaves to underground parts of potato plants was studied in greenhouse experiments. Thirty days after stem inoculation, 90% of plants expressed symptoms at the stem base and 95% of plants showed browning of internal stem tissue. The GFP-tagged Dickeya sp. was detected by dilution plating in extracts of the ...

Conflicts of interest between the sexes over control mating can be reflected in various aspects morphology and behaviour, including structure genitalia copulation duration. In Arrenurus water mites (Hydrachnidia: Arrenuridae) there are two main patterns sperm transfer that differ degree potential male uptake by females. some species, males equipped with an intromittent organ (the ‘petiole’) is ...