Expression of recombinant eukaryotic proteins in Escherichia coli often results in the formation of inclusion bodies. In the case of disulfide-bonded proteins such as hEGF, inclusion body formation can be anticipated if the protein is produced in the bacterial cytosol. The consequence is improper folding which results in aggregation. Proper folding and solubility of such protein are pre-requisi...

The in situ stimulation of Fe(III) oxide reduction by Geobacter bacteria leads to the concomitant precipitation of hexavalent uranium [U(VI)] from groundwater. Despite its promise for the bioremediation of uranium contaminants, the biological mechanism behind this reaction remains elusive. Because Fe(III) oxide reduction requires the expression of Geobacter's conductive pili, we evaluated their...

13 To understand the mechanisms underlying the increased N2O reductase activity in the 14 Bradyrhizobium japonicum 5M09 mutant from enrichment culture under N2O respiration, we 15 analyzed the expression of genes encoding denitrification reductases and regulators. Our 16 results suggest a common regulation of nap (encoding periplasmic nitrate reductase) and nos 17 (encoding N2O reductase). 18

An AND logic gate based on a synthetic quorum-sensing (QS) module was constructed in a Shewanella oneidensis MR-1 mtrA knockout mutant. The presence of two input signals activated the expression of a periplasmic decaheme cytochrome MtrA to regenerate the extracellular electron transfer conduit, enabling the construction of AND-gated microbial fuel cells.

Acidic mammalian chitinase (AMCase) has been shown to be associated with asthma in mouse models, allergic inflammation and food processing. Here, we describe an E. coli-expression system that allows for the periplasmic production of active AMCase fused to Protein A at the N-terminus and V5 epitope and (His)6 tag (V5-His) at the C-terminus (Protein A-AMCase-V5-His) in E. coli. The mouse AMCase c...

Gram-negative bacteria are surrounded by two membrane bilayers separated by a space termed the periplasm. The periplasm is a multipurpose compartment separate from the cytoplasm whose distinct reducing environment allows more efficient and diverse mechanisms of protein oxidation, folding, and quality control. The periplasm also contains structural elements and important environmental sensing mo...

Recombinant Escherichia coli systems expressing organophosphorous hydrolase (OPH) have been proposed for biotransformation of toxic organophosphate compounds. However, whole cell biocatalyst systems are critically disadvantaged due to substrate diffusion limitations. To enhance whole cell biocatalytic efficiency, we engineered E. coli, for the first time, to secrete metal ion cofactor-requiring...

Symbiotic N2 fixation is essential for the plant’s growth because it can fix reactive nitrogen compounds in soil. However, all steps of this process be hampered by several biotic and abiotic environmental factors. This study mainly focused on discussing impacts 12 major factors reviewing significant numbers research works. According to information from these works, we found some physiological g...