There are present in commercial peptones substances which exhibit bacteriostatic properties for certain bacterial species. These substances are bacteriostatic in the oxidized form, but not in the reduced form. Their bacteriostatic action can be overcome, and their concentration titrated, by the addition of reduced thiol compounds to the media in which they are present. Different brands of pepto...

Berkleasmium sp. Dzf12, an endophytic fungus from Dioscorea zingiberensis, is a high producer of spirobisnaphthalenes with various bioactivities. The exopolysaccharide (EPS) produced by this fungus also shows excellent antioxidant activity. In this study, the experimental designs based on statistics were employed to evaluate and optimize the medium for EPS production in liquid culture of Berkle...

Decolorization of azo dye Red 3BN by two bacterial species Bacillus cereus and B. megaterium has been analyzed using mineral effluent, consisting of known concentration of the dye in ZZ medium. Physico chemical parameters like carbon source, nitrogen source, temperature, pH and inoculum volume are optimized for the decolorization process by changing one parameter at a time. Optimal condition fo...

Medium composition and culture conditions for the acid protease production by Aspergillus niger I1 were optimized by response surface methodology (RSM). A significant influence of temperature, KH(2)PO(4), and initial pH on the protease production was evaluated by Plackett-Burman design (PBD). These factors were further optimized using Box-Behnken design and RSM. Under the proposed optimized con...

Enrofloxacin metabolism by Mucor ramannianus was investigated as a model for the biotransformation of veterinary fluoroquinolones. Cultures grown in sucrose-peptone broth were dosed with enrofloxacin. After 21 days, 22% of the enrofloxacin remained. Three metabolites were identified: enrofloxacin N-oxide (62% of the total absorbance), N-acetylciprofloxacin (8.0%), and desethylene-enrofloxacin (...

A tremorgenic mycotoxin was isolated from Penicillium paraherquei Abe ex G. Smith and identified as verruculogen. It was produced at the rate of approximately 1 mg/g of the dried fungal mycelium cultured on peptone-enriched Czapek-Dox medium at 28 degrees C.

The acid in the commonly used indol reagent described by Kovacs (1928, 1932) and Diagnostic Procedures and Reagents (1950) changes the reaction of the medium in which the indol production is tested and prevents the further observation of biochemical processes, killing the bacteria themselves. It was found that, by heating 0.1 ml. or less of culture (broth or peptone water), indol production by ...

A bacterial strain was isolated from dhal industry red gram waste and identified as Bacillus. A thermostable extracellular amylase was partially purified from the strain. Optimum temperature and pH for the enzyme were found to be 60°C and 6.5, respectively. The maximum amylase production was achieved with maltose as carbon source. Among the nitrogen sources, peptone and yeast extract produced m...

Instances of antagonism among the enteric group of bacteria have been frequently reported in the literature, but these are concerned primarily with mixed cultures in which many different factors, difficult to analyze, are involved. In 1925 Gratia observed a strain of Escherichia coli which produced a diffusible and thermolabile antibiotic substance. He called this substance "principe V." This "...

1. Many substances besides the specific broth filtrates of Besredka can be utilized as topical applications to protect guinea pigs from the effects of massive doses of staphylococcus given subcutaneously. (Plain broth, peptone 10 per cent, peptone 1 per cent, Liebig's meat extract, mustard plaster and normal horse serum.) 2. Where such protection occurs, no matter what the stimulus is, the loca...