The interaction affinity between human IgG and a short peptide ligand (hexameric HWRGWV) was investigated by following the shifts in frequency and energy dissipation in a quartz crystal microbalance (QCM). HWRGWV was immobilized by means of poly(ethylene glycol) tethered on QCM sensors coated with silicon oxide, which enhanced the accessibility of the peptide to hIgG and also passivated the sur...

background: prostate cancer after lung cancer is the second cause of cancer-associated death in men. in recent years, targeted therapy of cancer has attracted researchers attention. the targeted therapy lead to decrease in side effects of drugs. studies have indicated that targeting peptides for cancer cells represent valuable tools for diagnostic and therapeutic. recently, phage display peptid...

Recombinant adenoviruses have emerged as promising agents in therapeutic gene transfer, genetic vaccination, and viral oncolysis. Therapeutic applications of adenoviruses, however, would benefit substantially from targeted virus cell entry, for example, into cancer or immune cells, as opposed to the broad tropism that adenoviruses naturally possess. Such tropism modification of adenoviruses req...

Elabela/toddler is the second endogenous ligand recently identified after Apelin, that binds to G protein-coupled receptor APJ. Elabela a 54-amino acid peptide initially in fish and human genomes classified as noncoding. This precursor can be cleaved shorter sequences (32, 21, 11 amino acids), which bind activate APJ, blocked by APJ antagonists. Contrary Apelin widely distributed organs tissues...

One method for improving cancer treatment is the use of nanoparticle drugs functionalized with targeting ligands that recognize receptors expressed selectively by tumor cells. In theory such targeting ligands should specifically deliver the nanoparticle drug to the tumor, increasing drug concentration in the tumor and delivering the drug to its site of action within the tumor tissue. However, t...

We evaluate the potential of native mass spectrometry (MS) and ion mobility (IM-MS) for the screening of protein : ligand complexes when very subtle conformational changes are involved. As a proof of concept, we investigate the interactions between a peptide deformylase (PDF1B), a promising target for the development of new antibiotics, and three of its specific inhibitors that bind in differen...

milk proteins provide a natural source of bioactive peptides with potential health benefits and applications in the food industry. the release of these peptides from milk proteins is achieved either by hydrolysis using digestive proteases or by lactic acid bacteria fermentation. peptides, particularly those derived from milk proteins, can exert a wide range of nutritional, functional and biolog...

Ligand-dependent recruitment of coactivators to estrogen receptor (ER) plays an important role in transcriptional activation of target genes. Agonist-bound ER has been shown to adopt a favorable conformation for interaction with the LXXLL motifs of the coactivator proteins. To further examine the affinity and ligand dependence of the ER-coactivator interaction, several fluorescently tagged shor...

This study describes a form of partial agonism for a CD8+ CTL clone, S15, in which perforin-dependent killing and IFN-gamma production were lost but Fas (APO1 or CD95)-dependent cytotoxicity preserved. Cloned S15 CTL are H-2Kd restricted and specific for a photoreactive derivative of the Plasmodium berghei circumsporozoite peptide PbCS 252-260 (SYIPSAEKI). The presence of a photoactivatable gro...

This work validates an in vitro observation that silk peptides derived from the α-chymotrypsin digestion of fibroin heavy chain promote wound healing properties. A molecular level evidence of binding of these peptide fragments to growth factors, their receptors and the corresponding ligand-receptor complexes has been provided. The strengths of interactions and possible conformational changes ca...