Background and Aims: In this study we improved the purification of immunoglobulins from equine antiserum raised against the venom of 6 types of scorption species. Caprylic acid (octanoic acid), a fatty acid, was found to have no effect on the activity of the enzymes pepsin, which is used in antivenom purification to digest Fc fragment of immunoglobulins to obtain F(ab´)2. Materials and Methods...

An in vitro experiment based on the digestion of haemoglobin with pepsin was carried out to test the claim made by some manufacturers that the antacids used in their proprietary preparations had an intrinsic antipeptic activity independent of the change brought about by alteration of pH. In no case could these claims be substantiated.

Background The use of the microarray tool is an essential advantage in the allergy diagnosis process in patients with multiple sensitizations showing clinical patterns attributable to the underlying cross-reaction to a single or multiple panallergen. Among panallergens LTP is a thermostable and resistant to pepsin digestion allergen. This features makes it a potent food allergens and explains t...

I t has long been known that pepsin digestion occurs only in acid solution while trypsin digestion takes place in neutral or alkaline solutions. This marked influence of the reaction on the rate of hydrolysis is a characteristic property of enzymes in general. A great advance in the explanation of this phelaomenon was made by SSrensen (1) who was able to show in the case of a number of enzymes ...

Basement membrane procollagen biosynthesis was studied in organ cultures of embryonic rat parietal yolk sac endoderm by following [14C]proline incorporation into nondialyzable proteins. After reduction with 2-mercaptoethanol the 14C-proteins synthesized were characterized by agarose gel filtration and disc electrophoresis in the presence of sodium dodecyl sulfate. The labeled procollagen was id...

The protein constituents and thermal properties of hemp (Cannabis sativa L.) protein isolate (HPI) as well as 11Sand 7S-rich HPIs (HPI-11S and HPI-7S) were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and different scanning calorimetry (DSC), and their amino acid composition and in vitro digestibility were also evaluated, as compared to soy protein isola...

The effects of acid pepsin pretreatment, bile acids and reductants on the excystation of Clonorchis sinensis metacercariae were studied. The velocities of metacercarial excystation, digestion of the metacercarial outer layer and tryptic BAEE hydrolysis were measured in various media. Pretreatment with acid or acid pepsin accelerated the excystation, but was not required under adequate tryptic a...

The experiment was carried out using fistulated multiparous Holstein Friesian crossbred (75% Holstein Friesian and 25% Red Sindhi) dairy cows in their dry period fed on untreated rice straw to evaluate the nutritive value of local protein feed resources using the in sacco method and in vitro pepsin-pancreatin digestion. Experimental feeds were cottonseed meal (CSM); soybean meal (SBM); dried br...

BACKGROUND Avian influenza virus H5N1 has demonstrated considerable pandemic potential. Currently, no effective vaccines for H5N1 infection are available, so passive immunotherapy may be an alternative strategy. To investigate the possible therapeutic effect of antibody against highly pathogenic H5N1 virus on a mammal host, we prepared specific equine anti-H5N1 IgGs from horses vaccinated with ...

Experiments were performed to examine the relationship between the structure and function of ovine uterine serpin (OvUS). Limited proteolytic digestion of OvUS caused cleavage of the 55-57 kDa OvUS to a 42 kDa product nearly identical in molecular weight to a naturally-occurring breakdown product of OvUS. N-terminal amino acid sequencing and MALDI-MS revealed that, unlike other serpins, OvUS wa...