An in vivo strategy to apply the activation effect of acetyl-CoA on phosphoenolpyruvate carboxylase (PEPC) and pyruvate carboxylase (PYC) to increase succinate production in Escherichia coli was studied. This approach relies on the increased intracellular acetyl-CoA and CoA levels by overexpressing E. coli pantothenate kinase (PANK). The results showed that coexpression of PANK and PEPC, and PA...

Phosphoenolpyruvate carboxylase (PEPC) catalyses the initial fixation of atmospheric CO2 into oxaloacetate and subsequently malate. Nocturnal accumulation of malic acid within the vacuole of photosynthetic cells is a typical feature of plants that perform crassulacean acid metabolism (CAM). PEPC is a ubiquitous plant enzyme encoded by a small gene family, and each member encodes an isoform with...

Photosynthetic characteristics and induction of crassulacean acid metabolism (CAM) by drought stress were investigated in Sedum album, Sedum stoloniferum and Rosularia elymaitica from Crassulaceae. Titratable acidity, malate content, phosphoenolpyruvate carboxylase (PEPC) activity and gas exchange parameters were determined in plants at the end and beginning of the photoperiod. Results showed t...

The biosynthesis of Pep5, a lanthionine-containing antimicrobial peptide, is directed by the 20-kbp plasmid pED503. We identified a 7.9-kbp DNA-fragment within this plasmid which covers the information for Pep5 synthesis in the homologous host Staphylococcus epidermidis 5 which has been cured of pED503. This fragment contained, in addition to the previously described structural gene pepA and th...

Two novel phosphoenolpyruvate carboxylase (PEPC) isoforms have been biochemically characterized from endosperm of developing castor oil seeds (COS). The association of a 107 kDa PEPC subunit (p107) with an immunologically unrelated bacterial PEPC-type 64 kDa polypeptide leads to marked physical and kinetic differences between the PEPC1 p107 homotetramer and PEPC2 p107/p64 heterooctamer. COS p10...

The high rates of photosynthesis and the carbon concentrating mechanism (CCM) in C4 plants is initiated by enzyme phosphoenolpyruvate (PEP) carboxylase (PEPC). flow inorganic into CCM driven PEPC's affinity for bicarbonate (KHCO3 ), which can be rate limiting when atmospheric CO2 availability restricted due to low stomatal conductance. We hypothesize that natural variation KHCO3 across specific...

The crystal structure of phosphoenolpyruvate carboxylase (PEPC; EC 4. 1.1.31) has been determined by x-ray diffraction methods at 2.8-A resolution by using Escherichia coli PEPC complexed with L-aspartate, an allosteric inhibitor of all known PEPCs. The four subunits are arranged in a "dimer-of-dimers" form with respect to subunit contact, resulting in an overall square arrangement. The content...

photosynthetic characteristics and induction of crassulacean acid metabolism (cam) by drought stress were investigated in sedum album, sedum stoloniferum and rosularia elymaitica from crassulaceae. titratable acidity, malate content, phosphoenolpyruvate carboxylase (pepc) activity and gas exchange parameters were determined in plants at the end and beginning of the photoperiod. results showed t...