OBJECTIVE To compare genome-wide methylation profiles in maternal leukocyte DNA between normotensive and preeclamptic pregnant women at delivery. METHODS Age, body mass index matched case-control comparison of methylation at 27,578 cytosine-- guanine sites in 14,495 genes in maternal leukocyte DNA in women with preeclampsia (PE; n = 14) and normotensive controls (n = 14). RESULTS PE was ass...

The ability to detect and compensate for errors is crucial in producing effective, goal-directed behavior. Human error processing is reflected in two event-related brain potential components, the error-related negativity (Ne/ERN) and error positivity (Pe), but the functional significance of both components remains unclear. Our approach was to consider error detection as a decision process invol...

We have shown previously that enkephalin-containing peptides of high molecular weight increase 10- to 15-fold 3 days after adrenal denervation and have suggested that much of this material may represent newly synthesized proenkephalin. In this paper we report that most of the material appearing after denervation is indeed intact proenkephalin. The putative proenkephalin was partially purified b...

Eleven commercially available PE-labeled anti-human (IL-1-α, IL-6, IL-8, TNF-α, IL-17A, IL-5, IL-10, IL-12 and IL-13) and anti-mouse (IL-10, TNF-α) cytokine monoclonal antibodies (mAbs) were tested for cross-reactivity with cattle, goat, and sheep cytokines. Cross-reactivity was assessed by comparative analysis with the standard reactivity of the target species. Our data demonstrated that anti-...

The rat and mouse proenkephalin genes each contains two distinct promoters, one of which is utilized exclusively by spermatogenic cells. The germ cell-specific promoter lacks TATA sequences, is G+C rich, and contains multiple initiation sites. To investigate the nature of the cis-acting elements that determine selective transcription of the proenkephalin gene in male germ cells, two rat proenke...

Error detection is essential for monitoring performance and preparing subsequent behavioral adjustments, and is associated with specific neural responses in the anterior cingulate cortex (ACC). To investigate whether different brain mechanisms subserve the processing of commission vs. accuracy errors, we recorded EEG in adult participants while they performed a novel speeded GO-NOGO aiming task...

OBJECTIVE To develop a model for prediction of late preeclampsia (PE; which develops at or after 34 weeks' gestation) based on maternal history and characteristics, mean arterial pressure (MAP), and circulating levels of mRNA for the placenta-specific 1 (PLAC1) gene in maternal plasma at 14-18 weeks' gestation. METHOD This was a screening study of singleton pregnancies at 14-18 weeks' gestati...

Spermatogenic cells have been previously shown to be a major site of testicular proenkephalin gene expression. Using RNA gel-blot analysis of purified mouse and hamster germ cells and of testes from prepuberal and germ cell-deficient mutant mice, we now have demonstrated that, in addition to its previously described expression by somatic (Leydig) cells, the gene for a second opioid peptide prec...

We have investigated whether plasma exchange in myasthenia gravis synergises with additional immunosuppressive drug therapy (azathioprine, cyclophosphamide or cytosine arabinoside). Serum anti-acetylcholine receptor (AChR) antibody titres were followed over 28 days after a course of PE in 20 patients, of whom 17 were taking 20-80 mg prednisone on alternate days. No significant difference was ob...

School sports societies are organised with the goal to enable pupils to engage in their preferred sports. Also, this system is implemented in certain sports within school sports societies with the aim to facilitate better development of motor and functional abilities, and anthropometric characteristics. During training sessions of school sports societies, pupils should not be exposed to excessi...