نتایج جستجو برای: penicillin g acylase
تعداد نتایج: 453817 فیلتر نتایج به سال:
a Department of Biochemistry and Molecular Biology I, Faculty of Biology, University 7 Complutense of Madrid, Madrid, Spain; and b Department of Environmental Biology, Centro 8 de Investigaciones Biológicas, CSIC, Madrid, Spain 9 10 Running head: Approach to catalytic mechanism of Penicillin V acylase from Streptomyces 11 lavendulae 12 #Address correspondence to Isabel de la Mata, [email protected]...
Secretion of Escherichia coli penicillin acylase was improved by codon-based random mutagenesis of its signal peptide. The mutagenesis technology was applied to the gene region coding for positions Lys2 to Thr13 (N half) and Ala14 to Leu25 (C half) of the signal peptide. Protein secretion was higher in several signal peptide variants (up to fourfold with respect to the wild-type value).
6-Aminopenicillanic Acid Production by Intact Cells of E. coli Containing Penicillin G Acylase (PGA)
The alpha-amino acid ester hydrolase from Acetobacter turbidans ATCC 9325 is capable of hydrolyzing and synthesizing beta-lactam antibiotics, such as cephalexin and ampicillin. N-terminal amino acid sequencing of the purified alpha-amino acid ester hydrolase allowed cloning and genetic characterization of the corresponding gene from an A. turbidans genomic library. The gene, designated aehA, en...
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