The aim of this study was to apply RAPD technique to analyze the genetic variability among the Iranian CA-MRSA isolates.The RAPD amplification was implemented on 25 strains isolated from the anterior nares of 410 healthy children using four randomly selected oligonucleotide primers from the stocks available in our laboratory, including the primers 1254, GE6, OLP6 and OLP13 from our stock. The a...

Three shrimps population (Penaeus semesulacatus, Penaeus japonicus,Meta Penaeus monoceros) were investigated for determination of the genetic structure by using (PCR-RAPD) and SDS-PAGE. The results obtained from RAPD technique showed the same percentages of similarity (100%) within the three studied population. On the other hand, the degrees of similarity detected by SDSPAGE were 90%, 87% and 8...

The objective of this work was to compare the usefulness of a randomly amplified polymorphic DNA (RAPD) assay to that of the ATB ID32C kit (bioMérieux, France) for identification of different species of Candida isolated from clinical specimens. The RAPD-PCR patterns obtained with OPE-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed...

Typing methods utilising DNA technology were applied to a collection of Trichophyton mentagrophytes and T. rubrum isolates from skin and nail infections. The methods included restriction enzyme analysis (REA), hybridisation with the DNA probe poly (dG-dT), randomly amplified polymorphic DNA (RAPD) by PCR and restriction analysis of a segment of PCR-amplified rDNA. All these tests successfully d...


 The aim of this study was ‎the‎ discrimination Salmonella‎‎ isolated from chicken and their feed ‎and drinking water for the epidemiological control salmonellosis. Totally, 289 samples, ‎including 217 cloaca swabs, 46 water, 26 samples were collected five ‎different farms in Karbala governorate, Iraq. Conventional bacteriology tests, API 20E, Vitek 2, serology used bacterial identificati...

The random amplified polymorphic DNA (RAPD) assay was used to assess the level of DNA damage in various exposed and unexposed Culex quinquefasciatus larvae to acetone and chloroform extracts of Curcuma longa and Melia azedarach at different concentrations (6.25, 12.5 and 25 ppm). This is the first report of an analysis of genomic alterations in plant extracts-treated mosquito larvae using RAPD-...

Background and Objective: Escherichia coli bacteria are one of the most important bacterial races causing urinary tract infection, which is responsible for 90% causes infection originated in human intestine, this Gram- Negative facultative aerobic that moves by peripheral flagella belongs to Enterobacteriaceae.
 Materials methods: The were isolated from UTI, after they diagnosed using ligh...

Lasiodiplodia theobromae adalah cendawan patogen penting dan kosmopolitan di daerah tropis subtropis. Kisaran tanaman inangnya sangat luas, termasuk komoditas bernilai ekonomi tinggi Indonesia, seperti jeruk, kakao, karet, pisang, manggis. Keragaman intraspesies galur hasil isolasi lima diamati berdasarkan pada ciri morfologi marka molekuler RAPD-PCR. ditunjukkan dari perbedaan laju pertumbuhan...

A total of 500 local chickens from different regions Iraq, which were divided into five groups according to the they taken, namely, East (E), West (W), North (N), South (S), and Central (C) Iraq. The blood samples collected tubes containing an EDTA, DNA extraction was carried. Using 20 primers Gen Script USA, random amplification polymorphic (RAPD-PCR) carried out. reaction PCR had a final volu...