DNA size markers are widely used to estimate the size of DNA samples on agarose or polyacrylamide gelelectrophoresis (PAGE). DNA markers can be prepared by mixing PCR products with definite sizes.Alternatively, they are prepared by restriction enzyme digestion of the genomic DNA of bacteriophages ornatural and synthetic DNA plasmids. The present study describes engineering of ...

in this study, the genetic diversity among paecilomyces variotti isolaes was investigated. sampling was performed from pistachio garden in seven geographical region of kerman province during 2011-2012. seventy isolates were identified as p. variotii species using morphological criteria. the genetic diversity among 20 selected isolates was assayed by variation at ssr marker bands using 12 pair p...

Radish is a root vegetable crop, and many F1 cultivars have been developed by exploiting heterosis. Considerable time resources are required to test the performance combining ability of parental lines; therefore, economical methods identify them needed before field tests. This study was conducted determine minimum number markers elite lines. PCR-based were applied radish lines selected based on...

For the diagnosis of unexplained male infertility a multiplex PCR for 6 markers, which are well-known as candidate genes for studying male infertility and located on the human Y-chromosome, has been designed. The multiplex PCR products have been separated on a 12 channel microchip electrophoresis system, which can analyze different samples simultaneously. By combining the technologies of multip...

Exsheathing fluid (EF) and excretory-secretory products (ES) of infective third-stage cultured larvae ofOstertagia circumcincta were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE). Five and seven predominant proteins were found in the EF and ES products, respectively. Immunoblotting by sheep pre-infection serum did not react with any of the EF and ES proteins, b...

BACKGROUND Current methods for molecular-based diagnosis of disease rely heavily on modern molecular biology techniques for interrogating the genome for aberrant DNA sequences. These techniques typically include amplification of the target DNA sequences followed by separation of the amplified fragments by slab gel electrophoresis. As a result of the labor-intensive, time-consuming nature of sla...

AIMS This study evaluates the microbial ecology of 'Alheira' by traditional microbiological analysis and a PCR-denaturing gradient gel electrophoresis (DGGE) protocol. METHODS AND RESULTS Total microbial DNA from 'Alheiras' was extracted directly from the products and subjected to PCR using Eubacterial primers for 16S rDNA. The amplicons were separated by DGGE. The results demonstrated that d...

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...

1Institute for Biochemistry and Protein Research, Zlnstitute for Molecular Genetics, Agricultural Biotechnology Center, H-2101, GOdOll6, Hungary Microsatellites are tandemly repeated short (1-6 bp) simple sequences that are highly abundant in most eukaryotic genomes. (1-4) Because of the variation in the number of repeats, these microsatellites show extensive length polymorphism. Thus, they are...

Denaturing Gradient Gel Electrophoresis is a influential and feasible device for sequence examination of the variety of complicted natural microbial populations. For PCR amplification of 16S rDNA products and specific primers were used and analyzed by denaturing gel electrophoresis in gradient. Probes specific for the cluster Genusand were designed to bind to sequences within the region amplifi...