نتایج جستجو برای: pcr mycoplasma
تعداد نتایج: 187962 فیلتر نتایج به سال:
Mycoplasma species, which can cause upper respiratory tract disease (URTD), are significant pathogens of birds, mammals, fish, and reptiles. Mycoplasmosis is of high concern for chelonian conservation, with the most well-documented cases in gopher and desert tortoises. Mycoplasma sp. infections have been reported in captive and free-living box turtles ( Terrapene spp.). We documented URTD assoc...
BACKGROUND Mycoplasma mastitis is increasingly posing significant impact on dairy industry. Although the effects of major conventional mastitis pathogens on milk components has been widely addressed in the literature, limited data on the effects of different Mycoplasma and Acholeplasma spp. on milk quality and quantity is available. The aim of this study was to determine the casual relationship...
For the first time, a total of 14 Mycoplasma isolates were cultured from 24 moribund prawns investigated during an outbreak of mid-crop mortality syndrome in northern Queensland, Australia. Mycoplasma were isolated from the gill appendages, brains and eyes of the prawns. Mycoplasma growth occurred between 20 and 37°C with or without CO2 in modified Frey's medium containing 0.5 to 3.0% sodium ch...
Ureaplasma, spp. Mycoplasma genitalium, and Mycoplasma hominis are associated with infection of the genitourinary tract, reproductive failure, and neonatal morbidity and mortality. We have developed a multiplex PCR for the detection of these Mycoplasmatales in a single amplification reaction. The analytical sensitivities of this assay were 10.8, 10.8, and 8.8 CFU for each organism, respectively...
A molecular survey of hemoplasma (Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum') in Yamaguchi Prefecture and surrounding areas was performed by using molecular methods. PCR-RFLP with HindIII revealed that 2 cats were infected with M. haemofelis, and 16 with 'C. Mycoplasma haemominutum' among 102 randomly selected cats. Partial 16S rRNA gene sequences of M. haemofelis and 'C. My...
mycoplasma capricolum subsp capricolum (mcc) is one of the etiological agents of contagious agalactia(c.a) in goats which can cause significant economic losses. the aim of this study was to detect mcc from goats of qom province in iran. a total of 111 samples were collected from suspected goats to c.a and cultured in pplo broth supplemented for mcc isolation. the bacteria dnas were extracted fr...
The intergenic spacer region between the 16S and 23S rRNA genes of Mycoplasma haemomuris, previously classified as 'Haemobartonella muris', was amplified by PCR and sequenced for analysis of the primary and secondary structures of the RNA transcript. The spacer region consisted of 219 base-pairs and lacked the spacer tRNA gene. A hypothetical secondary structure predicted in the RNA transcript ...
BACKGROUND Mycoplasma synoviae is an important avian pathogen which can cause both respiratory disease and synovial joint inflammation (synovitis) in poultry. Mycoplasmas spp. may cause the respiratory system infection in ostriches with symptoms such as inflammation of the nose, trachea and also damages of lungs. OBJECTIVES The current study aimed to use the M. synoviae specific Polymerase Ch...
bacterial vaginosis or non-specific vaginitis describes the disease caused by a change in the normal flora of the vagina, which leads to the elimination of lactobacilli, generating hydrogen peroxide and excess growth of bacteria, particularly anaerobic bacteria. this disease is the most prevalent infection of the female genital tract, and the rate of frequency of anaerobic bacteria, specificall...
Background and purpose: Misabortion is a disorder resultant of numerous causes such as infections. It is clear that bacterial infections due to genital mycoplasma, Listeria and brucella can cause septic abortion. A right time diagnosis of these infections can improve women fertility. This study was conducted to survey the efficiency of PCR in detecting the specimens with negative culture res...
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