نتایج جستجو برای: pcr multiplex

تعداد نتایج: 186945  

Journal: :Methods 2001
C T Wittwer M G Herrmann C N Gundry K S Elenitoba-Johnson

The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...

2016
Qiu Yang Yongyu Rui

Nosocomial infections caused by Acinetobacter spp. resistant to carbapenems are increasingly reported worldwide. Carbapenem-resistant Acinetobacter (CRA) is becoming a serious concern with increasing patient morbidity, mortality, and lengths of hospital stay. Therefore, the rapid detection of CRA is essential for epidemiological surveillance. Polymerase chain reaction (PCR) has been extensively...

Journal: :archives of clinical infectious diseases 0
abdolaziz rastegar lari antimicrobial resistance research center, tehran university of medical sciences, tehran, ir iran abdollah karimi pediatric infectious research center (pirc) of shahid beheshti university of medical scieces, tehran, ir iran fatemeh fallah infectious diseases and tropical medicine research center, shahid beheshti university of medical sciences tehran, ir iran; infections diseases and tropical medicine research center, faculty of medicine shahid beheshti university of medical sciences,tehran, ir iran goli angoti msc student of microbiology, department of microbiology. shahid beheshti university of medical sciences, tehran, ir iran anahita sanaei pediatric infectious research center (pirc) of shahid beheshti university of medical scieces, tehran, ir iran leila azimi antimicrobial resistance research center, tehran university of medical sciences, tehran, ir iran

results we found 28 cases with positive results for b. melitensis by multiplex pcr technique which was significantly different from of sat (p<0.05). six samples were positive for b. abortus by pcr. conclusion the results of present study showed that multiplex pcr assay is a rapid and sensitive technique for diagnosis of brucellosis compared to sat. however it is more accurate when coupled with ...

ژورنال: طب جنوب 2014
احمری, غلامرضا, ارجمندزادگان, محمد, دهقان, فاطمه, ذوالفقاری, محمدرضا, کسروی, علیرضا, کلانتری, سالومه, گراوند, سمیه,

زمینه: تشخیص آلودگی میکروبی آب شرب به‌روش کشت علاوه بر هزینه بری، زمان‌بر بوده و بر حسب میزان آلودگی چند روز به‌طول می‌انجامد. این درحالی است که شهروندان در این زمان از آب مورد بررسی استفاده می‌نمایند. روش‌های ملکولی دارای حساسیت و سرعت عمل بالایی و زمان عملیاتی کمتری هستند. در این تحقیق روش Multiplex PCR جهت ارزیابی سریع میکروبی آب از نظر کلی فرم، Ecoli و تشخیص احتمالی باکتری‌های VBNC در آزمای...

Journal: :iranian journal of basic medical sciences 0
seyed mahmoud amin marashi department of microbiology &amp; immunology, babol university of medical sciences, babol, iran bahram nasr esfahani department of microbiology, isfahan university of medical sciences, isfahan, iran akbar tavakoli department of microbiology, isfahan university of medical sciences, isfahan, iran sharareh moghim department of microbiology, isfahan university of medical sciences, isfahan, iran mohammad reza pourshafie department of microbiology, pasteur institute of iran, tehran, iran mansoor salehi departments of genetics, isfahan university of medical sciences, isfahan, iran

objective(s) amongst the various antibiotic resistant elements in vibrio. cholerae, sxt constin (sxt-c) is important. we were going to design a quick method for determination of antibiotic resistance gene pattern in sxt-c. materials and methods ninety four v. cholerae o1el tor isolates were used in this study. antibiotic susceptibility testing, multiplex pcr amplification of sxt-c containing df...

2012
Seyed Mahmoud Amin Marashi Bahram Nasr Esfahani Akbar Tavakoli Sharareh Moghim Mohammad Reza Pourshafie Mansoor Salehi

OBJECTIVES Amongst the various antibiotic resistant elements in Vibrio. cholerae, SXT constin (SXT-C) is important. We were going to design a quick method for determination of antibiotic resistance gene pattern in SXT-C. MATERIALS AND METHODS Ninety four V. cholerae O1 El Tor isolates were used in this study. Antibiotic susceptibility testing, multiplex PCR amplification of SXT-C containing d...

Journal: :BioTechniques 2007
John W Coleman J Erik Johnson David K Clarke

Researchers carrying out quantitative, multiplex, realtime PCR and RT-PCR require master mixes that provide accurate, simultaneous quantification of multiple targets in the same reaction without time-consuming optimization. Our results at Wyeth Research show that QuantiTect® Multiplex Kits from QIAGEN are well suited for multiplex analysis of up to four RNA targets in two-step and onestep real-...

Journal: :Journal of microbiology and biotechnology 2012
Mi Kyung Lee Hye Ryoun Kim

In April 2009, the H1N1 pandemic influenza virus emerged as a novel influenza virus. The aim of this study was to compare the performances of several molecular assays, including conventional reverse transcription polymerase chain reaction (RT-PCR), two real-time reverse transcription (rRT)-PCRs, and two multiplex RTPCRs. A total of 381 clinical specimens were collected from patients (223 men an...

2014
Zhiyuan Wu Yunqing Zhang Xinju Zhang Xiao Xu Zhihua Kang Shibao Li Chen Zhang Bing Su Ming Guan

A multiplex snapback primer system was developed for the simultaneous detection of JAK2 V617F and MPL W515L/K mutations in Philadelphia chromosome- (Ph-) negative myeloproliferative neoplasms (MPNs). The multiplex system comprises two snapback versus limiting primer sets for JAK2 and MPL mutation enrichment and detection, respectively. Linear-After exponential (LATE) PCR strategy was employed f...

Journal: :Letters in applied microbiology 2011
S Kumar A C Udaya Shankar S C Nayaka O S Lund H S Prakash

AIMS To develop a highly sensitive and rapid protocol for simultaneous detection and differentiation of Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) in pepper and tomato. In this study, we use the multiplex PCR technique to detect dual infection of these two viruses. METHODS AND RESULTS A multiplex RT-PCR method consisting of one-tube reaction with two primer pairs targeted to re...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید