نتایج جستجو برای: pcr detection

تعداد نتایج: 709127  

Background and Aims: High prevalence of Methicillin Resistant Staphylococcus Aureus isolates (MRSA) as well as the multi-drug resistance in this bacterium causes difficulties in the treatment of infections due to these bacteria. Hence, detection of MRSA isolates by rapid and accurate methods is necessary. PCR-ELISA is an accurate and molecular technique that is used for the detection of several...

Journal: :Applied and Environmental Microbiology 1999

Journal: :Nippon Ishinkin Gakkai Zasshi 1994

Journal: :Brazilian Journal of Microbiology 2012

A Kavand , L Sadeghi , M Naderpour , Z Nouri ,

Background and Aims: Plant certification programs need reliable, fast, cheap and sensitive methods for detection of systemic pathogens with special interest in virus and viroid detection. Reverse transcriptase-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certification of plant propagating materials. The main object of the present study was the optimization ...

Background and purpose: There are various methods for molecular detection of SARS-CoV2 genome among which, PCR-based methods are the most reliable for making diagnosis. The majority of approved PCR kits for detection of Coronavirus are based on TaqMan real-time PCR which is expensive due to incorporating fluorescent and quencher-harboring probe. The aim of this study was to design a simple and ...

Journal: :iranian journal of microbiology 0
mehrdad ravanshad department of virology, faculty of medical sciences, tarbiat modares university, tehran, iran. z khanlari department of virology, faculty of medical sciences, tarbiat modares university, tehran, iran. m rasouli department of immunology, prof. alborzi clinical microbiology research center, shiraz university of medical sciences, shiraz, iran. m ziyaeyan department of immunology, prof. alborzi clinical microbiology research center, shiraz university of medical sciences, shiraz, iran. s falahi department of virology, faculty of medical sciences, tarbiat modares university, tehran, iran.

background and objectives: pcr is a sensitive assay and could be used as an accurate diagnostic method for detecting various types of microorganisms' genome in low concentration in biological specimens. the demand for sensitive, rapid, safe and easy detection of pcr products has led researchers to a combination of this method with elisa. materials and methods: conserved sequences were selected ...

D. Morshedi M. R. Sadeghi R. Kargar Moakhar R. Salehi Tabar S. A. Ghorashi, S. S. Ghaemmaghami

Marek’s disease (MD) is a lymphoproliferative disease of chickens characterized by lymphocyticinfiltration of various organs. The present study was an attempt to use polymerase chain reaction (PCR) tooptimize a rapid and reliable assay for detection of MDV genome. Detection of serotype 1 of MDV (MDV-1)was confirmed by presence of a 200 bp DNA fragment as a PCR product. Differentiation of MDV-1 ...

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