Background: Ovarian carcinoma is one of the leading causes of cancer-related death among females. K-ras codon 12 mutations are commonly occurring mutations in different types of cancers and leads to resistance against anti-EGFR therapeutics. Hence, determination of mutations in k-ras gene is crucial for predicting response to anti-EGFR therapies. This study aimed to evaluate the prevalence of k...

Tilapia is an economically important fish in the world. High mortality caused by low temperature a serious problem to tilapia farming during overwintering. In this study, genome-wide significant QTL interval, located on chrLG18, controlling cold-tolerance trait was identified full-sib family of GIFT originated line using ddRAD-seq-based mapping. The explained 21.5% phenotypic variances. Six pai...

Abstract Objectives Human identification systems based on STRs are widely used in human population genetics and forensic analysis. This study aimed to validate the cross-reactivity of three known human-specific STR i.e. GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS PowerPlex® Fusion 6C chimpanzee. Results The present revealed successful amplification 18 loci using 20 system. marker...

BACKGROUND Universal nuclear protein-coding locus (NPCL) markers that are applicable across diverse taxa and show good phylogenetic discrimination have broad applications in molecular phylogenetic studies. For example, RAG1, a representative NPCL marker, has been successfully used to make phylogenetic inferences within all major osteichthyan groups. However, such markers with broad working rang...

Gibberella zeae is one of the most devastating pathogens of barley and wheat in the United States. The fungus also infects noncereal crops, such as potatoes and sugar beets, and the genetic relationships among barley, wheat, potato, and sugar beet isolates indicate high levels of similarity. However, little is known about the toxigenic potential of G. zeae isolates from potatoes and sugar beets...

In textile industry, marker planning is one of the main operations in the cutting fabric stage. Marker packing is usually used to maximize cloth exploitation and minimize its waste. In this research, a method is used based on new found meta-heuristic imperialist competitive algorithm (ICA) and Bottom-Left-Fill Algorithm (BLF) to achieve optimal marker packing. Function of the proposed method wa...

PURPOSE We undertook a systematic approach to identify breast cancer (BC) marker genes with molecular assays and evaluated these marker genes for the detection of minimal residual disease in peripheral blood mononuclear cells (PBMCs). EXPERIMENTAL DESIGN We used serial analysis of gene expression to identify a range of genes that were expressed in BC but absent in the expression profiles of b...

MAS (marker assisted selection) is useful for cultivar development in the lower plant Saccharina japonica . In this study, 167 screened SSR markers and 125 individuals were used to construct a S.japonica genetic linkage map with 27 groups total Haldane length of 2069 cM. Constructed aligned assembled pseudo-chromosomes S. genome sequences (NCBI: MEHQ00000000) anchored 23 31 pseudo-chromosomes. ...