Rice false smut (RFS) is an important rice disease that is caused by the pathogen Ustilaginoidea virens. In this study, we developed a real-time polymerase chain reaction (PCR) assay to detect U. virens and to estimate the level of disease. The genomic DNAs of U. virens and rice were extracted together from the rice samples. Real-time PCR assays were performed and compared to conventional neste...

The present study was aimed at evaluating the role of a real-time polymerase chain reaction (RT-PCR) assay in the diagnosis of tuberculous meningitis (TBM) and pulmonary tuberculosis (PTB) and comparison of its performance with the in-house conventional PCR (C-PCR) assay. A RT-PCR assay using SYBR green methodology and C-PCR targeting a segment of the gene for mycobacterial IS6110 region were e...

introduction: telomeric repeat amplification protocol assay (trap assay) is a standard established technique to detect the activity (expression) of telomerase in the cancerous samples. it includes a time-consuming procedure that has significant drawbacks such as high cost, complicacy, time-consuming procedure, equipment-dependent steps and false-positive signals caused by inclusion of pcr insid...

We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were evaluated with this system. Of the 34 culture-positive specimens, 31 were PCR positive, and 60 of the culture-negative specimens were PCR negative. An internal standard has bee...

Research has demonstrated that swine feed can be a fomite for viral transmission and additives reduce contamination. Therefore, the objective of this study was to evaluate two in contaminated with PEDV or PRRSV. Feed included: no treatment, 0.33% commercial formaldehyde-based product, 0.50% medium chain fatty acids (MCFA) blend. samples were inoculated PRRSV alone together at an inoculation con...

Exserohilum rostratum was the major cause of the multistate outbreak of fungal meningitis linked to contaminated injections of methylprednisolone acetate produced by the New England Compounding Center. Previously, we developed a fungal DNA extraction procedure and broad-range and E. rostratum-specific PCR assays and confirmed the presence of fungal DNA in 28% of the case patients. Here, we repo...

Previously, we designed an internally controlled quantitative nested real-time (QNRT) PCR assay for Mycobacterium tuberculosis DNA in order to rapidly diagnose tuberculous meningitis. This technique combined the high sensitivity of nested PCR with the accurate quantification of real-time PCR. In this study, we attempted to improve the original QNRT-PCR assay and newly developed the wide-range Q...

Background: Bacteremia due to Enterococcus faecalis is usually caused by strains resistant to most antibiotics. Effective management of the disease is dependent on rapid detection and characterization of the bacteria, and determination its sensitivity pattern to antimicrobial drugs. The aim of this study was to investigate a more rapid and reliable assay for simultaneous diagnosis of enterococc...