Background Osteoarthritis (OA) is the most common joint disease characterized by progressive degradation of articular cartilage, synovial hyperplasia, bone remodeling and angiogenesis [1]. Symptomatic painful OA leads to psychological distress significant restrictions in daily living. Shortening work life accompanied an exponential rise healthcare costs causes become a major socio-economic burd...

Increased oxidative stress in the human brain is observed neurodegenerative diseases such as Parkinson’s disease (PD) and Alzheimer’s (AD), considered to be a major cause of progression these states. A very long-chain fatty acid, nervonic acid (NA), main found various sphingolipid species central nervous system. NA plays an important role forming plasma membrane’s lipid bilayer maintaining norm...

This study aims to investigate the inhibitory effects of oridonin nanosuspension on human prostatic carcinoma PC-3 cell line in vitro. The PC-3 cells were incubated with increasing concentrations of oridonin solution and nanosuspensions for 12 hours, 24 hours, and 36 hours. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay was performed to measure cellular viability and i...

Monoclonal antibody (MAb) 2H1, raised in mice immunized with membrane fractions from cultured rat pheochromocytoma cells (clonal line PC-12), detects a polypeptide from rat brain and PC-12 cell membranes of 60-65 KD apparent molecular mass. The polypeptide has been localized by immunoelectron microscopy in the rough endoplasmic reticulum (RER) of neurons. By light microscopic immunocytochemistr...

We previously demonstrated that the AT2 receptor is a glycoprotein containing N-linked oligosaccharide side chains and that the marked disparity between the sizes of AT2 receptors from different tissues was related to different degrees of N-glycosylation. In the present study, we used an inhibitor of N-glycosylation, tunicamycin, as well as an endoglycosidase, glycopeptidase-F, to examine the c...

OBJECTIVES In the present study, we investigated whether the pH of IP6 could influence its anti-tumoral activity in vitro. METHODS PC-3 cells were exposed to IP6 at pH 5, pH 7, and pH 12 and we evaluated the metabolic activity (WST-1 assay), cell proliferation (cell count), cell cycle distribution (FACS), and mitochondrial depolarization (JC-1 staining) in vitro. RESULTS Our results demonst...