A thermophilic, spore-forming rod isolated from hay compost in Korea was subjected to a taxonomic study. The micro-organism, designated strain SK-1(T), was identified as being aerobic, Gram-positive, motile and rod-shaped. Growth of the isolate was observed at 45-70 degrees C (optimum 60 degrees C) and pH 6.0-9.0 (optimum pH 7.5). The G+C content of the genomic DNA was 43.9 mol%. Chemotaxonomic...

Sulfakinins (SKs) are a family of multifunctional neuropeptides that have been shown to have myotropic activity on muscles of the digestive system and to function as feeding satiety factors. Here, we confirm via cloning the presence of two sulfakinins (Rhopr-SK-1 and Rhopr-SK-2) in Rhodnius prolixus. Reverse transcriptase quantitative PCR demonstrates that the Rhopr-SK transcript is highly expr...

Small conductance Ca²⁺-activated K⁺ (SK) and intermediate conductance Ca(2+)-activated K⁺ (IK) channels are thought to be involved in detrusor smooth muscle (DSM) excitability and contractility. Using naphtho[1,2-d]thiazol-2-ylamine (SKA-31), a novel and highly specific SK/IK channel activator, we investigated whether pharmacological activation of SK/IK channels reduced guinea pig DSM excitabil...

Crosses heterozygous and homozygous for Sk-1, Sk-2 and Sk-3 were examined by light microscopy. All three Spore killers behave similarly. In heterozygous killer x sensitive crosses, meiosis and ascospore development are normal until after the second postmeiotic mitosis when four of the eight ascospores in each ascus stop developing and degenerate. The four surviving ascospores carry the killer. ...

We describe a method to ligate a PCR-amplified DNA fragment with T-protruding cassettes, which have multiple sites for endonuclease, promoter sequences of T3 and T7, and annealing sites for the universal M13 forward and reverse primers. This method, which we named T-cassette ligation, substantially facilitated direct sequencing and subcloning of PCR products. Two T-cassettes with a protruding T...

In this paper, we consider the asymptotic properties of the numbers of spanning trees and Eulerian trials in circulant digraphs and graphs. Let C(p, s1, s2, . . . , sk) be a directed or undirected circulant graph. Let T (C(p, s1, s2, . . . , sk)) and E(C(p, s1, s2, . . . , sk)) be the numbers ∗This work was partially supported by the Natural Science Foundation of China, email: fjzhang@jingxian....

Let S be a finite set. Let k be a positive integer. Let A be a subset of Sk satisfying |A| = |S|k−1. Let B = Sk \ A. For every v ∈ Sk and every i ∈ {1, 2, . . . , k}, we denote by vi the i-th component of the k-tuple v (remember that v is an element of Sk, that is, a k-tuple of elements of S). Then, every v ∈ Sk satisfies v = (v1, v2, . . . , vk). Let F be the set of all pairs (a, b) ∈ A × B fo...

BACKGROUND Streptokinase is the most widely used thrombolytic agent and can now be made using recombinant DNA technology. The present trial was initiated to assess an indigenous recombinant streptokinase (Shankinase, r-SK). AIM To compare the efficacy and safety of indigenous recombinant streptokinase (Shankinase, r-SK) and natural streptokinase (Streptase, n-SK). SETTINGS AND DESIGN Double...

when s1, . . . , sk are positive integers and z a complex number in the unit disk. For k = 1, this is the classical polylogarithm Lis (z). These multiple polylogarithms can be defined also in terms of iterated Chen integrals and satisfy shuffle relations. Multiple polylogarithms in several variables are defined for si ≥ 1 and |zi | < 1(1 ≤ i ≤ k) by Li(s1,...,sk)(z1, . . . , zk) = ∑ n1>n2>···>n...