The genome of Sapovirus (SaV), a causative agent of gastroenteritis in humans and swine, contains either two or three open reading frames (ORFs). Functional motifs characteristic to the 2C-like NTPase (NTPase), VPg, 3C-like protease (Pro), 3D-like RNA-dependent RNA polymerase (Pol), and capsid protein (VP1) are encoded in the ORF1 polyprotein, which is afterwards cleaved into the nonstructural ...

Sheeppox virus from an outbreak of sheeppox that occurred in Srinagar (Jammu and Kashmir, India) in 2000 was isolated by inoculation of susceptible sheep and further re-isolated in cell culture. The field virus, adapted to grow in lamb testes culture, was evaluated for its potential use as challenge virus in potency testing of sheeppox vaccine currently in use. The virus (passage 6) produced se...

PURPOSE Cell death is an essential process in normal development and homeostasis. In eyes, corneal epithelial injury leads to the death of cells in underlying stroma, an event believed to initiate corneal wound healing. The molecular basis of wound induced corneal stromal cell death is not understood in detail. Studies of others have indicated that ceramide may play significant role in stromal ...

Cancer is a complex, multi-factorial, multi-stage disease and a global threat to human health. Early detection of nature and stage of cancer is highly crucial for disease management. Recent studies have proved beyond any doubt about the involvement of the ubiquitous, myriad ligand binding, multi-functional human protein, hyaluronan-binding protein 1 (HABP1), which is identical to the splicing f...

Previous studies on resting cell metabolism of microorganisms relating to p32 incorporation into the intact cell have been carried out by Hotchkiss (1956) for micrococci, lactobacilli, and streptococci, and by Juni et al. (1948) for yeast. These studies in the main were concerned with turnover of various intracellular phosphoruscontaining compounds during conditions under which the organism was...

The incorporation of radiophosphorus (P”“) into desoxyribonucleic acid (DNA) has been shown to parallel the incidence of mitosis in various tissues (14). In tissues without mitotic activity, such as brain cortex, skeletal muscle, and cartilage, injected P32 was not found in DNA (4). Accordingly, it is generally thought that the presence of P32 in DNA occurs as a result of the synthesis of addit...

Abstract Background The diseases caused by the Capripoxvirus species have very similar symptoms and are difficult to distinguish clinically. According a recent report, not strictly host specific. Objective This study aimed identify viruses from ovine (include sheep goat) or bovine, which will assist in selecting appropriate vaccine correct measures control diseases. Method Universal primers for...

The localization and abundance of the estrogen receptor activation factor (E-RAF) and a small nuclear ribonucleoprotein (snRNP) complex containing three proteins, p32, p55 and p60, which interact with the nuclear estrogen receptor II (nER II), have been studied in rat endometrial epithelial cells by means of immunofluorescence and high resolution quantitative immunocytochemistry. In the cytopla...

We determined the prevalence of Y chromosome deletions in a population of 60 Brazilian nonobstructive azoospermic and severely oligozoospermic men. PCR-based screening of microdeletions was performed on lymphocyte DNA for the presence of 14 sequence-tagged sites (STS) located in the azoospermic factor (AZF) on the Yq chromosome. All STS were amplified efficiently in samples from 12 fertile men ...