BACKGROUND AND OBJECTIVES Positive immunostaining for the tumor suppressor protein p16 is associated with the presence of mucosal or αsubtypes of human papillomavirus (HPV) in cervical and genital squamous cell carcinoma (SCC). The aim of this study was to determine whether p16 immunostaining is also associated with mucosal HPV in extragenital SCC. MATERIAL AND METHODS Paraffin sections of le...

Background: Expression of the tumor suppressor p16 increases during aging and replicative senescence. Methodology/Principal Findings: Here, we report that the microRNA miR-24 suppresses p16 expression in human diploid fibroblasts and cervical carcinoma cells. Increased p16 expression with replicative senescence was associated with decreased levels of miR-24, a microRNA that was predicted to ass...

BACKGROUND Inactivation of the tumour suppressor gene p16 (CDKN2/MTS-1/INK4A) and K-ras mutations are among the most frequent genetic alterations in human malignancies. AIMS To investigate the tumour suppressor gene p16 and its possible association with K-ras mutations in intrahepatic cholangiocarcinomas of the liver. METHODS The status of p16 was evaluated in 41 cholangiocarcinomas by meth...

Dysfunctional telomeres limit cellular proliferative capacity by activating the p53-p21- and p16(INK4a)-Rb-dependent DNA damage responses (DDRs). The p16(INK4a) tumor suppressor accumulates in aging tissues, is a biomarker for cellular senescence, and limits stem cell function in vivo. While the activation of a p53-dependent DDR by dysfunctional telomeres has been well documented in human cells...

PURPOSE It has been suggested that p16 has a role in glucocorticoid (GC)-related apoptosis in leukemic cells, but the exact mechanisms have yet to be clarified. We evaluated the relationship between the GC response and p16 expression in a lymphoma cell line. METHODS We used p16 siRNA transfection to construct p16-inactivated cells by using the B-cell lymphoblast cell line NC-37. We compared g...

BACKGROUND p16 expression is a well established biomarker of cervical dysplasia and carcinoma arising from high risk human papilloma virus infection. Increased p16 expression is also seen in squamous neoplasms arising at other sites, including head, neck, and oropharyngeal tract. Squamous lesions are also frequently encountered at ocular surface and peri-orbital skin sites, but the prevalence o...

The incidence of HPV associated Head and Neck Squamous Cell Carcinoma (HNSCC) is increasing over the past 30 years. It is a growing public health concern. It has been reported that tissues of HPV associated HNSCCs over express p16 INK4a . Therefore p16 INK4a is used as a surrogate marker to detect HPV associated HNSCC. Immunohistochemical detection of p16 INK4a is an easy and simple technique t...

viable cell concentration and luminescence activity of p. stutzeri p16 luxab4 was measured byplate counting and luminometery in sterile and non-sterile soil adjusted to matric potential of -1500, -750 and-30 kpa. results showed that matric potential had a significant effect on survival and activity of p. stutzerip16 luxab4 in soil. p. stutzeri p16 luxab4 could survive better in hutner’s minimal...

The combined impact of mutations in p16 and p53 was examined in cellular growth, transformation, and tumor formation. In cultured cells, p16 loss enhanced growth at high density and conferred susceptibility to oncogene-induced transformation. In vivo, mice doubly deficient for p16 and p53 showed an increased rate of tumor formation with particular susceptibility to aggressive angiosarcomas. Fur...

Pituitary tumors develop at a high frequency in retinoblastoma (Rb)-knockout mice; however, defects in the Rb gene are not common in human pituitary tumors. The inverse correlation of Rb and p16 defects in certain human tumors has led us to investigate the expression of p16 in human pituitary tumors as an indirect mechanism of Rb inactivation. By Western blot analysis, the p16 gene product was ...