Induction of UDP-glucuronosyltransferases (UGTs) toward thyroxine (T4) and p-nitrophenol (pNP) by 3-methylcholanthrene (MC), dexamethasone (DEX), clofibrate (Cl), and MC combined with DEX or Cl was studied in rat hepatocyte culture. We have developed a sensitive method for the measurement of glucuronide conjugates of the two substrates based on HPLC analysis of culture medium. MC, Cl, or DEX in...

The cytochrome P450 enzymes (P450s) that mediate mammalian xenobiotic metabolism are highly versatile monooxygenases, which show wide and overlapping substrate ranges but generally poor catalytic rates. Re-engineering of these P450s may enable the development of useful biocatalysts for industrial applications. In the current study, restriction enzyme-mediated DNA family shuffling was used to cr...

Treatment of anhydrous FeX2 (X = Cl, Br) with 1 equiv. of the asymmetric chiral PNP pincer ligands PNP-R,TAD (R = iPr, tBu) with an R,R-TADDOL (TAD) moiety afforded complexes of the general formula [Fe(PNP)X2]. In the solid state these complexes adopt a tetrahedral geometry with the PNP ligand coordinated in κ(2)P,N-fashion, as shown by X-ray crystallography and Mössbauer spectroscopy. Magnetiz...

A sensitive screen-printed electrode modified with multi-walled carbon nanotubes (MWCNTs/SPE) was applied to determine simultaneously m-nitrophenol, o-nitrophenol and p-nitrophenol. The electrochemical response showed that o-nitrophenol, m-nitrophenol and p-nitrophenol were entirely separated at the MWCNTs/SPE interface. Under the optimized conditions, it was found that the detection limits wer...

The study focused on investigating the solvent adsorption of p-Nitrophenol (PNP) onto activated carbons for wastewater treatment. It explored influence adsorbate concentration and adsorbent size equilibrium isotherms removal rates to develop efficient processes. examined under conditions utilizing both Langmuir Double-Langmuir models Dubinin–Radushkevich equation. Remarkably, all demonstrated e...

We consider the question whether two queries to SAT are as powerful as one query. We show that if PNP[1] = PNP[2] then Locally either NP = coNP or NP has polynomial-size circuits. PNP = PNP[1]. p2 p2=1. p2 = UPNP[1] \RPNP[1]. PH = BPPNP[1]. Moreover we extend work of Hemaspaandra, Hemaspaandra and Hempel to show that if P p2 [1] = P p2 [2] then p2 = p2 . We also give a relativized world where P...

INTRODUCTION Chronic obstructive pulmonary diseases (COPD) have some systemic effects including systemic inflammation, nutritional abnormalities, skeletal muscle dysfunction, and cardiovascular, skeletal and neurological disorders. Some studies have reported the presence of peripheral neuropathy (PNP) at an incidence of 28-94% in patients with COPD. Our study aimed to identify whether PNP affec...

The biodegaradation of p-nitrophenol was studied experimentally at different initial p-nitrophenol concentrations under different environmental and operational conditions (like incubation temperature, pH, inoculum size etc.) by using microbial strain Arthrobacter chlorophenolicus A6. The rate of degradation of pnitrophenol was observed maximum at incubation temperature 30 ̊C and pH 7 ± 0.2, inoc...

There is a high level of conservation tryptophans within the active site architecture cellulase family, whereas function four in catalytic domain Cel7A unclear. By mutating tryptophan residues from Penicillium piceum (PpCel7A), binding affinity between PpCel7A and p-nitrophenol-D-cellobioside (pNPC) was reduced as determined by Michaelis–Menten constants, molecular dynamics simulations, fluores...

The effects of pentaand hexadecanoic acids on the population and orientational structure of p-nitrophenol, at the airlwater interface at a bulk pH of 2, have been studied by using the technique of second-harmonic generation. The results indicate that, with increasing fatty acid at the interface, (1) the population of p-nitrophenol at the airlwater interface first increases and then decreases, (...