Porcine cytochrome P450 (CYP) 1A2 was purified to electrophoretic homogeneity from the hepatic microsomes of beta-naphthoflavone-treated male pigs. In a reconstituted system, this enzyme showed a good catalytic activity towards caffeine, acetanilide, and methoxyresorufin, all known markers of mammalian CYP1A2. Using 3'- and 5'-rapid amplification of coding DNA (cDNA) ends (RACE), we amplified f...

This paper presents the synthesis of some new theophylline derivatives with potential bronchodilatatory properties. The compounds were synthesized starting from theophylline and 8-R-theophyllines (R=Br, NO2, pyrrolidinyl, piperidinyl, morpholinyl, imidazolyl) that were reacted with 4-(2,3-epoxy-propyloxy)-acetanilide. The new compounds, which have not been mentioned in the literature concerning...

The cation-p interaction is a general, strong, noncovalent binding force that is used throughout nature. The side chains of the aromatic amino acids [phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp)] provide a surface of negative electrostatic potential than can bind to a wide range of cations through a predominantly electrostatic interaction. In this brief overview, the fundamental na...

The method of Bruton and Hartley for the isolation of short NH2-terminal sequences of peptides has been applied to /3-galactosidase. Maleyl-P-galactosidase was hydrolyzed with thermolysin and the digest was acidified. After passage through Dowex 50, Thr-Met was obtained from the eluate. This procedure has been extended. For the isolation of a larger NH&erminal peptide, maleyl-protein was treate...

Field trials were conducted during the 2019-2020 cropping season to assess efficacy of S-metolachlor and flumetsulam (triazolopyrimidine sulfonanilide)+S-metolachlor (chloro-acetanilide) pre-emergence herbicides in controlling weeds maize at Chisumbanje estate. The experiment was laid out as a randomised complete block design (RCBD) with three treatments replicated thrice. Treatments used inclu...

Several workers have provided evidence that deacetylation occurs both in vivo and in vitro. Abbott (1942) found that, when N-acetylglycine and sodium benzoate were administered orally to rabbits, the increase in hippuric acid excretion was only slightly less than when the corresponding amount of glycine was given with the benzoate. Elson, Goulden & Warren (1946) found that the rat deacetylated ...

The effect of repeated ingestion of caffeine on rat-liver microsoma Jenzymes was studied. In the rat, ingestion of 20-24 mg caffeine/kg/day in the drinking water for more than 2 wk inhibited aminopyrine N-demethylase activity. This loss of activity approached 60 ~o at the end of an 8-wk dosing schedule. Acetanilide-hydroxylating activity of rat-liver microsomal enzyme was initially stimulated, ...