Antimicrobial resistance has emerged as a major danger to contemporary medicine around the world. Carbapenems are highest class of B-lactam drugs which considered most effective and safest antibiotics available. Increasing spread carbapenemases been noted across world restricts treatment options. The aim this study was determine incidence carbapenem resistant genotypic pathways in tertiary care...

Three selective chromogenic culture media (CHROMagars ESBL, CTX-M and KPC) were evaluated for their ability to support the growth of nine Klebsiella pneumoniae isolates producing OXA-48 carbapenemase in combination with other β-lactamases. CHROMagar ESBL and CHROMagar KPC were the most sensitive media, supporting growth of all isolates with a detection limit as low as < 100 CFU/ml. Five isolate...

Service de Bactériologie-Virologie, INSERM U914 ‘Emerging Resistance to Antibiotics’, Hôpital de Bicêtre, Assistance Publique/ Hôpitaux de Paris, Faculté de Médecine et Université Paris-Sud, 78 rue de Général Leclerc, K.-Bicêtre, France; Service de Bactériologie, Hopital Européen Georges Pompidou, Université Paris-Descartes, Paris, France; Department of Microbiology, Faculty of Medicine, Kuwait...

The gene encoding the carbapenemase OXA-181 (an OXA-48 variant) was identified from a Citrobacter freundii isolate coproducing NDM-1. The whole sequence of plasmid pT-OXA-181 bearing the blaOXA-181 gene was determined and revealed a 84-kb mobilizable but non-self-conjugative IncT-type plasmid. It totally differs from the 7.6-kb ColE-type and blaOXA-181-bearing plasmid recently identified in a K...

A lethal peritonitis model was induced in mice with a Klebsiella pneumoniae isolate producing the carbapenemase OXA-48. Administration of a single dose (up to 100 mg/kg) of the antibiotic piperacillin-tazobactam, imipenem-cilastatin, ertapenem, or cefotaxime had little or no impact on lethality. Ceftazidime had the highest efficacy in vivo, which mirrored its in vitro activity; this was not the...