PCR Conditions: PCR reactions are carried out in a total volume of 25 fi\ containing: 25 ng genomic DNA, 5 pmole of each primer, 1.5 mM MgCl2, 200 jiM dNTPs, 50 mM tris-Cl, 20 mM (NH^SO,,, pH 9, 0.25 mM spermidine and 0.6 units of ReplinaseTM (NEN/Dupont). Amplification was performed in 96 well plates in a BiosyclerTM oven (Bios Corp., New Haven, CT) for 32 cycles. We used a 4 temperature cycle...

Air pollution in Poland and particularly in Silesia is among the worst in Europe. Many coal mines and coke oven plants are located in this area, representing a major source of carcinogenic polycyclic aromatic hydrocarbons (PAHs). We quantitated the PAH exposure level in air samples using personal sampling devices, collected urine samples from the same individuals, and measured 1-hydroxypyrene w...

The danger excessive coking pressure poses to the integrity and longevity of coke ovens has been recognized for many years [1-7]. During carbonisation in a coke oven fissures in the coke are generated due to stresses that arise from the differential contraction rates in adjacent layers of coke, which are at different temperatures [8]. Typically they are longitudinal, i.e. perpendicular to the o...

in order to improve food texture, hydrocolloids are used widely as gelling material in food systems. wild sage seed has remarkable amounts of gum (mucilage) with good functional properties. this mucilage also improves the rheological, textural and functional properties of foods. in this study the effects of different drying methods including hot air drying (40, 50, 60, 70 and 80°c), freeze dryi...

BACKGROUND Quince has many health benefits. Dried quince has been used as a tea for centuries. The aim of this study was to investigate the influences of two drying methods on the antioxidant activity of the quince. MATERIAL AND METHODS Fifty two fresh Iranian quinces (Cydonia oblonga) were obtained from different parts of Shiraz. Half of the quinces were peeled and both peel and flesh immedi...

Variables related to oven-drying samples of cheese and cheese products to determine moisture content were examined to provide more efficient and reproducible methods. Over 6500 samples of cheese were analyzed in an effort to modify the current AOAC procedure. The gravity atmospheric oven was unsuitable for use in accurate moisture analysis because of wide temperature differentials within the ov...

White blood cell DNA adducts were measured in coke oven workers, in residents from the area next to the coke oven in Silesia, Poland (highly industrialized region), and in residents from the rural area of Poland using the 32P-postlabeling technique. This method detected aromatic adducts including adducts formed by polycyclic aromatic hydrocarbons (PAHs). Highest levels of adducts in DNA were se...

AIMS To determine the association between lung function of coke oven workers and exposure to coke oven emissions. METHODS Lung function data and detailed work histories for workers in recovery coke ovens of a steelworks were extracted from a lung function surveillance system. Multiple regressions were employed to determine significant predictors for lung function indices. The first sets of lu...

OBJECTIVE To investigate the application of BPDE-albumin adducts as monitoring biomarkers for coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) and to explore possible relationship between BPDE-albumin adducts and urinary 1-hydroxypyrene (1-OHP) levels in them. METHODS Thirty-seven coke oven workers from a coke plant and 47 controls without the occupational exposure to PAHs...