Growing next generation sequencing (NGS) throughput and new, sophisticated multiplexing strategies have significantly increased the number of samples users can prepare. The demand for reproducible and reliable data requires consistency between libraries. To achieve this, the VERSA Mini NGLP, liquid handling workstation has been optimized for NGS library preparation. Using NEXTflexTM library pre...

In microbial food safety, molecular methods such as quantitative PCR (qPCR) and next-generation sequencing (NGS) of bacterial isolates can potentially be replaced by diagnostic shotgun metagenomics. However, the methods for pre-analytical sample preparation are often optimized for qPCR, and do not necessarily perform equally well for qPCR and sequencing. The present study investigates, through ...

Cross-linked protein crystals (CLPCs) are very useful materials in applications such as biosensors, catalysis, and X-ray crystallography. Hence, preparation of CLPCs is an important research direction. During the preparation of CLPCs, an often encountered problem is that cracks may appear in the crystals, which may finally lead to shattering of the crystals into small pieces and cause problem i...

We present experimental results on a method to perform polarimetry on ensembles of single photons. Our setup is based on a measurement method known to be optimal for estimating the state of two level systems. The setup has no moving parts and is sensitive to weak sources (emitting single photons) of light as it relies on photon counting and has potential applications in both classical polarizat...

It is always desirable to achieve maximum sample clean-up, extraction, and pre-concentration with the minimum possible organic solvent. The miniaturization of sample preparation devices was successfully demonstrated by packing 10 mg of 11 electrospun polymer nanofibers into pipette tip micro column and mini disc cartridges for efficient pre-concentration of 1-hydroxypyrene in urine samples. 1-h...

An optimized fast vertical inter-mobility anchor point (MAP) handover scheme for Hierarchical Mobile IPv6 (HMIPv6) is proposed for heterogeneous wireless access networks. Two existing non-optimized vertical handover schemes – the simple vertical inter-MAP handover for HMIPv6 (SVH6) and fast vertical inter-MAP handover for HMIPv6 (FVH6) – assisted by existing hierarchical handover and fast hando...

Working with delicate tissue can be a complicating factor when performing immunohistochemical assessment. Here, we present a method that utilizes a ring-supported hydrophilized PTFE membrane to provide structural support to both living and fixed tissue during immunohistochemical processing, which allows for the use of a variety of protocols that would otherwise cause damage to the tissue. First...

In this study, the optimized method for designing IgG-binding magnetosomes based on integration of IgG-binding fusion proteins into magnetosome membrane in vitro is presented. Fusion proteins Mbb and Mistbb consisting of magnetosome membrane protein MamC and membrane associating protein Mistic from Bacillus subtilis as anchors and BB-domains of Staphylococcus aureus protein A as IgG-binding reg...

State preparation of quantum systems is a prerequisite for studying fundamental aspects of quantum measurement theory @1#, as well as for encoding quantum information @2# and its processing ~computing! @3#. Problems of state preparation have been dealt with most extensively in the realm of cavity quantum electrodynamics @4–8#. One proposal for state preparation of a cavity field mode @8# relies...